AB195028

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]

Name: Alexa Fluor® 488 conjugated Anti-Von Willebrand Factor antibody [EPSISR15] (ab195028) | Abcam
Brand: Abcam Limited
SKU: AB195028
Price: 575 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(8 Publications)

Anti-Von Willebrand Factor antibody [EPSISR15] - Alexa Fluor® 488 conjugated (ab195028) is a rabbit recombinant monoclonal antibody detecting Von Willebrand Factor in Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

ab195028 staining Von Willebrand Factor in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195028 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

Overlay histogram showing HepG2 cells stained with ab195028 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195028, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in HepG2 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Carrier free

Anti-Von Willebrand Factor antibody [EPSISR15] - BSA and Azide free
Unconjugated

Anti-Von Willebrand Factor antibody [EPSISR15]
660 APC

APC Anti-Von Willebrand Factor antibody [EPSISR15]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Von Willebrand Factor antibody [EPSISR15]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Von Willebrand Factor antibody [EPSISR15]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Von Willebrand Factor antibody [EPSISR15]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15]
HRP

HRP Anti-Von Willebrand Factor antibody [EPSISR15]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR15

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/alexa-fluor-488-rabbit-igg-monoclonal-epr25a-isotype-control-ab199091'>ab199091</a> - Rabbit monoclonal IgG (Alexa FluorÂ® 488), is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (ab195028) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPSISR15 is also available pre-conjugated to a variety of labels for your convenience – Anti-Von Willebrand Factor Alexa Fluor® 488 [EPSISR15] (ab195028).

Other related products
We have a range of other formats of antibody clone [EPSISR15] also available for your convenience: ab154193, Alexa Fluor® 488 - ab195028, Alexa Fluor® 647 - ab195029, HRP - ab195030, Carrier free - ab232473, APC - ab310828, PE - ab310904, Alexa Fluor® 594 - ab311713, Alexa Fluor® 568 - ab312989, Alexa Fluor® 555 - ab313194

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

See full target information VWF

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70707 PubMed40028673

2025

Secretomes From Non-Small Cell Lung Cancer Cells Induce Endothelial Plasticity Through a Partial Endothelial-to-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Bourreau,Emilie Navarro,Marine Cotinat,Morgane Krejbich,François Guillonneau,Catherine Guette,Alice Boissard,Cécile Henry,Isabelle Corre,Lucas Treps,Nicolas Clere

Bioengineering (Basel, Switzerland) 11: PubMed39329689

2024

Mesenchymal Stem Cell-Conditioned Media-Loaded Microparticles Enhance Acute Patency in Silk-Based Vascular Grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine L Lorentz,Ande X Marini,Liza A Bruk,Prerak Gupta,Biman B Mandal,Morgan V DiLeo,Justin S Weinbaum,Steven R Little,David A Vorp

Biological research 57:65 PubMed39261966

2024

The effect of CGRP and SP and the cell signaling dialogue between sensory neurons and endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Leroux,Micaela Roque,Elina Casas,Jacques Leng,Christelle Guibert,Beatrice L'Azou,Hugo Oliveira,Joëlle Amédée,Bruno Paiva Dos Santos

Cell biology international 48:290-299 PubMed38100125

2023

14-3-3-η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhou,Xiaopei Sun,Yi Dai,Xiaotong Wang,Zhihong Dai,Xiuli Li

Experimental and therapeutic medicine 22:1415 PubMed34676008

2021

Adipose mesenchymal stem cells and gingival mesenchymal stem cells have a comparable effect in endothelium repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Yang,Dongmei Xie,Wanwen Lin,Peng Xiang,Chaoquan Peng

eLife 10: PubMed34232859

2021

Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Eugenia Dieterle,Carles Solà-Riera,Chunyan Ye,Samuel M Goodfellow,Eva Mittler,Ezgi Kasikci,Steven B Bradfute,Jonas Klingström,Rohit K Jangra,Kartik Chandran

Thrombosis research 163:105-116 PubMed29407621

2018

Endothelial cell functions impaired by interferon in vitro: Insights into the molecular mechanism of thrombotic microangiopathy associated with interferon therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Jia,Craig Thelwell,Paula Dilger,Chris Bird,Sarah Daniels,Meenu Wadhwa

Stem cell research 25:202-212 PubMed29156376

2017

Comparative transcriptomic analysis of endothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiugong Gao,Jeffrey J Yourick,Robert L Sprando

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

Product promise