Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]

2 product images

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (ab195028)

  Overlay histogram showing HepG2 cells stained with ab195028 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195028, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  This antibody gave a positive signal in HepG2 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (ab195028)

  ab195028 staining Von Willebrand Factor in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195028 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).