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AB195028

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]

5

(1 Review)

|

(4 Publications)

Anti-Von Willebrand Factor antibody [EPSISR15] - Alexa Fluor® 488 conjugated (ab195028) is a rabbit recombinant monoclonal antibody detecting Von Willebrand Factor in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

Overlay histogram showing HepG2 cells stained with ab195028 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195028, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in HepG2 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

ab195028 staining Von Willebrand Factor in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195028 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-Von Willebrand Factor antibody [EPSISR15]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Von Willebrand Factor antibody [EPSISR15]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Von Willebrand Factor antibody [EPSISR15]

  • 660 APC

    APC Anti-Von Willebrand Factor antibody [EPSISR15]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Von Willebrand Factor antibody [EPSISR15]

  • HRP

    HRP Anti-Von Willebrand Factor antibody [EPSISR15]

  • Carrier free

    Anti-Von Willebrand Factor antibody [EPSISR15] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15]

  • 578 PE

    PE Anti-Von Willebrand Factor antibody [EPSISR15]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR15

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/alexa-fluor-488-rabbit-igg-monoclonal-epr25a-isotype-control-ab199091'>ab199091</a> - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.</p>" } } }
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You may be interested in:

AB108918

Human Von Willebrand Factor ELISA Kit (VWF)

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0 Reviews

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (ab195028) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPSISR15 is also available pre-conjugated to a variety of labels for your convenience – Anti-Von Willebrand Factor Alexa Fluor® 488 [EPSISR15] (ab195028).

Other related products
We have a range of other formats of antibody clone [EPSISR15] also available for your convenience: ab154193, Alexa Fluor® 488 - ab195028, Alexa Fluor® 647 - ab195029, HRP - ab195030, Carrier free - ab232473, APC - ab310828, PE - ab310904, Alexa Fluor® 594 - ab311713, Alexa Fluor® 568 - ab312989, Alexa Fluor® 555 - ab313194

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the gene VWF is important for maintaining hemostasis. It promotes platelet adhesion at sites of vascular injury by forming a molecular bridge between the sub-endothelial collagen matrix and the platelet-surface receptor complex GPIb-IX-V. Additionally, it acts as a chaperone for coagulation factor VIII, delivering it to the injury site, stabilizing its heterodimeric structure, and protecting it from premature clearance from plasma. This supplementary information is collated from multiple sources and compiled automatically.
See full target information VWF
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1415 PubMed34676008

2021

Adipose mesenchymal stem cells and gingival mesenchymal stem cells have a comparable effect in endothelium repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Yang,Dongmei Xie,Wanwen Lin,Peng Xiang,Chaoquan Peng

eLife 10: PubMed34232859

2021

Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Eugenia Dieterle,Carles Solà-Riera,Chunyan Ye,Samuel M Goodfellow,Eva Mittler,Ezgi Kasikci,Steven B Bradfute,Jonas Klingström,Rohit K Jangra,Kartik Chandran

Thrombosis research 163:105-116 PubMed29407621

2018

Endothelial cell functions impaired by interferon in vitro: Insights into the molecular mechanism of thrombotic microangiopathy associated with interferon therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Jia,Craig Thelwell,Paula Dilger,Chris Bird,Sarah Daniels,Meenu Wadhwa

Stem cell research 25:202-212 PubMed29156376

2017

Comparative transcriptomic analysis of endothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiugong Gao,Jeffrey J Yourick,Robert L Sprando
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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