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AB225404

Alexa Fluor® 488 Anti-WTAP antibody [EPR18744]

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(1 Publication)

Rabbit Recombinant Monoclonal WTAP antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0105, WTAP, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, WT1-associated protein, Wilms tumor 1-associating protein, hFL(2)D

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-WTAP antibody [EPR18744] (AB225404)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-WTAP antibody [EPR18744] (AB225404)

ab225404 staining WTAP in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225404 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18744

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WTAP also known as Wilms tumor 1-associated protein is a core component of the writer complex that catalyzes N6-methyladenosine (m6A) methylation on mRNA. With a molecular mass of approximately 39 kDa WTAP localizes almost ubiquitously in human tissues but shows higher expression in liver and kidney cells. This protein assists in the regulation of mRNA splicing and stability functioning as an adapter for RNA binding proteins.
Biological function summary

This protein plays a critical role in post-transcriptional gene regulation by being part of the m6A methyltransferase complex which includes other important proteins like METTL3 and METTL14. WTAP does not bind RNA directly but helps its partners achieve target specificity during gene expression processes. The presence of WTAP within the complex stabilizes and coordinates the activity of the entire methyltransferase assembly.

Pathways

The m6A methylation WTAP facilitates affects various pathways including mRNA splicing and degradation. This process intricately connects WTAP to the regulation of the JAK-STAT signaling pathway where its activity influences inflammatory responses and cell proliferation. Additionally WTAP has ties to the Wnt/β-catenin pathway collaborating with proteins like METTL3 to regulate gene expression involved in cell fate determination and embryogenesis.

Research shows WTAP has connections to various cancers such as hepatocellular carcinoma due to its role in mRNA processing and stability. This association often involves its interaction with other oncogenic proteins like METTL3 leading to aberrant gene expression patterns. WTAP also has relevance in acute myeloid leukemia where it influences mRNA maturation and impacts cell differentiation and proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed : 24316715, PubMed : 24407421, PubMed : 24981863). Acts as a mRNA splicing regulator (PubMed : 12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed : 17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed : 17095724).
See full target information WTAP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadv8640 PubMed40512859

2025

Interaction between NF-κB and PLAC8 impairs autophagy providing a survival advantage to prostate cells transformed by cadmium.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Shukla,Ashish Tyagi,Balaji Chandrasekaran,Bhawna Tyagi,Balpreet Singh,Thulasidharan Nair Devanarayanan,Venkatesh Kolluru,Murali K Ankem,Chendil Damodaran
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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