Alexa Fluor® 488 Anti-YKL-40/CHI3L1 antibody [EPR27396-56]
- BOND RX™ Validated
- RabMAb
- Recombinant
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Rabbit Recombinant Monoclonal YKL-40/CHI3L1 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.
View Alternative Names
Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB314164)](https://content.abcam.com/products/images/alexa-fluor-488-ykl-40-chi3l1-antibody-epr27396-56-ab314164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420927.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB314164)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling YKL-40/CHI3L1 with ab314164 at 1/100 (5.0 µg/ml). Negative control : no staining on human skeletal muscle. The section was incubated with ab314164 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB314164)](https://content.abcam.com/products/images/alexa-fluor-488-ykl-40-chi3l1-antibody-epr27396-56-ab314164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420925.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB314164)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling YKL-40/CHI3L1 with ab314164 at 1/100 (5.0 µg/ml). Membranous staining on crypt epithelial cells in human tonsil. The section was incubated with ab314164 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB314164)](https://content.abcam.com/products/images/alexa-fluor-488-ykl-40-chi3l1-antibody-epr27396-56-ab314164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420926.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB314164)
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling YKL-40/CHI3L1 with ab314164 at 1/100 (5.0 µg/ml). Positive staining on human glioma. The section was incubated with ab314164 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
Related conjugates and formulations (2)
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Anti-YKL-40/CHI3L1 antibody [EPR27396-56]
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Anti-YKL-40/CHI3L1 antibody [EPR27396-56] - BSA and Azide free
Complementary Products
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.
Pathways
YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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