Rabbit Recombinant Monoclonal CHIL3 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ym-1 + Ym-2 antibody [EPR15263] (AB205490), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-ym-1-ym-2-antibody-epr15263-ab205490--immunocytochemistry-immunofluorescence-img120646.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ym-1 + Ym-2 antibody [EPR15263] (AB205490)")
Publications
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- American journal of respiratory cell and molecular biology 64:629-6402021Altered Macrophage Function Associated with Crystalline Lung Inflammation in Acid Sphingomyelinase Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoanna M Poczobutt,Andrew M Mikosz,Christophe Poirier,Erica L Beatman,Karina A Serban,Fabienne Gally,Danting Cao,Alexandra L McCubbrey,Christina F Cornell,Kelly S Schweitzer,Evgeny V Berdyshev,Irina A Bronova,François Paris,Irina PetrachePubMed 33662226
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min) |
Species Mouse | Dilution info 1/100 | Notes This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
Target data
Function
Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy.
Additional Targets
Chil4
Alternative names
Chi3l3, Ym1, Chil3, Chitinase-like protein 3, Beta-N-acetylhexosaminidase Ym1, Chitinase-3-like protein 3, ECF-L, Eosinophil chemotactic cytokine, Secreted protein Ym1
Recommended products
Rabbit Recombinant Monoclonal CHIL3 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15263
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ym-1 and Ym-2 also known as chitinase-like proteins exhibit mechanical roles in the immune system without enzymatic chitinase activity. Ym-1 with a mass of approximately 43 kDa and Ym-2 are expressed predominantly in macrophages especially within inflammatory and allergic contexts. Expression is induced by IL-4 and IL-13 cytokines. Both proteins may also show presence in neutrophils and other innate immune cells during particular immune responses marking them important players in immune modulation.
- Biological function summary
Ym-1 and Ym-2 regulate tissue repair and remodeling. They play an important role in cell proliferation and matrix formation which is actively involved in tissue homeostasis and wound healing. These proteins are not typically found as part of a larger complex but may interact with multiple signaling molecules in the cell's microenvironment. Their functions in shaping the extracellular matrix and facilitating cell migration make them significant in resolving inflammation.
- Pathways
Ym-1 and Ym-2 are involved in the Th2 immune response and arginase pathways. They are associated with the regulation of Th2 cytokine production a pathway interconnected with adaptive immune responses to parasitic infections and allergic reactions. Ym proteins align with arginase pathways important in tissue repair and fibrosis and often correlate with Arginase-1 especially during inflammation and healing processes.
- Associated diseases and disorders
Ym-1 and Ym-2 connect predominately to asthma and fibrosis. Elevated levels observed in these conditions indicate their involvement in disease progression. In asthma Ym proteins modulate airway inflammation and remodeling collaborating with proteins like TGF-β to influence fibrosis and extracellular matrix deposition. The interaction between Ym proteins and such growth factors outlines their participation in pathological conditions offering potential targets for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab205490), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-ym-1-ym-2-antibody-epr15263-ab205490--immunocytochemistry-immunofluorescence-img120646.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab205490)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab205490)
ab205490 staining Chitinase 3 like protein 3 + Chitinase 3 like protein 4 in RAW246.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205490 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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