AB199814

Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal YY1 antibody - conjugated to Alexa Fluor® 488. Nucleus marker. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

INO80S, YY1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker (AB199814)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker (AB199814)

ab199814 staining YY1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker (AB199814)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker (AB199814)

Overlay histogram showing HeLa cells stained with ab199814 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab199814, 1/5000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Carrier free

Anti-YY1 antibody [EPR4652] - BSA and Azide free (Detector)
Unconjugated

Anti-YY1 antibody [EPR4652] - Nuclear Loading Control
Carrier free

Anti-YY1 antibody [EPR4652] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4652

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YY1 also known as Yin Yang 1 is a transcription factor that regulates gene expression by binding to specific DNA sequences. It is a protein with a molecular mass of approximately 68 kDa and is ubiquitously expressed in many tissues including liver lung and kidney. Functionally YY1 can both activate and repress transcription making it versatile in regulating gene activity. It interacts with several co-factors and other proteins influencing chromatin structure and gene expression.

Biological function summary

Yin Yang 1 plays an important role in various cellular processes such as cell proliferation differentiation and apoptosis. It is part of a chromatin remodeling complex where it assists in modifying the architecture of chromatin to control gene activity. Due to its ability to bind to diverse DNA motifs YY1 is involved in embryonic development and cellular differentiation. Additionally it interacts with other transcription factors to coordinate the expression of genes critical for cell cycle progression and survival.

Pathways

The Yin Yang 1 transcription factor is integrally involved in several key signaling pathways. It plays a notable role in the Wnt signaling pathway which is vital for controlling cell growth and differentiation. YY1 interacts with proteins such as β-catenin an important player in the Wnt pathway to mediate its transcriptional effects. Furthermore it also functions within the mTOR signaling pathway associating with proteins like mTOR itself to impact cell metabolism and growth.

Alterations in the expression or function of YY1 have been implicated in several conditions. Dysregulation of YY1 has strong associations with cancer as its interactions with proteins like MYC can lead to abnormal cell proliferation and tumor development. Additionally its role in neuronal differentiation links it with neurological disorders such as Alzheimer's disease. In these contexts YY1's interaction with other transcription factors and regulatory proteins influences disease progression and severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site (PubMed : 15329343, PubMed : 17721549, PubMed : 24326773, PubMed : 25787250). Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity (PubMed : 15329343, PubMed : 17721549, PubMed : 24326773, PubMed : 25787250). The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes (PubMed : 15329343, PubMed : 17721549, PubMed : 24326773, PubMed : 25787250). Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression (PubMed : 15329343, PubMed : 17721549, PubMed : 24326773, PubMed : 25787250). For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence (PubMed : 1655281). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed : 15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed : 15329343). May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed (PubMed : 11158321). Involved in DNA repair (PubMed : 18026119, PubMed : 28575647). In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation (PubMed : 28575647). Recruits the PR-DUB complex to specific gene-regulatory regions (PubMed : 20805357).. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.

See full target information YY1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 52: PubMed37232339

2023

Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junlong Yang,Liu Wang,Zhongjie Zhang,Qing Sun,Yuan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker (AB199814)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker (AB199814)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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