AB202509

Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368]

Name: Alexa Fluor® 555 conjugated Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509) | Abcam
Brand: Abcam Limited
SKU: AB202509
Price: 680 USD
Availability: InStock
Rating: 5 (6 reviews)

Recombinant
KO Validated
Advanced Validation
RabMAb
What is this?

(6 Reviews)

(23 Publications)

Anti-alpha smooth muscle Actin antibody [EPR5368] - Alexa Fluor® 555 conjugated (ab202509) is a rabbit recombinant monoclonal antibody detecting alpha smooth muscle Actin in ICC/IF, mIHC. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

3 Images

Lab

Immunocytochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

ab202509 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202509 at at 5µg/ml (shown in magenta) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in green) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

mIHC

Collaborator

Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

Immunofluorescence analysis of human lung adenocarcinoma labelled with ab202509. Image was acquired using Cell DIVE Multiplexed Imaging Solution (Leica Microsystems).

Formalin fixed paraffin embedded (FFPE) sections were incubated with ab202509 at 1/100 dilution, for 1h at room temperature. Nuclear DNA was labelled with DAPI (Red). Deparaffinization, antigen retrieval, biomarker labeling and imaging was performed following Cell DIVE recommended standard protocol. Cell DIVE automatically processed the image to perform autofluorescence removal, distortion correction, blank glass subtraction, flat-field correction, and stitching.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

ab202509 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202509 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368]
660 APC

APC Anti-alpha smooth muscle Actin antibody [EPR5368]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368]
578 PE

PE Anti-alpha smooth muscle Actin antibody [EPR5368]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [EPR5368]
Unconjugated

Anti-alpha smooth muscle Actin antibody [EPR5368]
Carrier free

Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free
Carrier free

Anti-alpha smooth muscle Actin antibody [EPR5368] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5368

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Mouse, Rat, Human, Human, Mouse, Rat

Applications

ICC/IF, IHC-P, mIHC, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/100", "mIHC-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 555 (ab202509) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists
Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 555 (ab202509) has over 5 independent reviews from customers.

Related products
Antibody clone EPR5368 is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha smooth muscle Actin Alexa Fluor® 555 [EPR5368] (ab202509).

Specificity confirmed
The specificity of Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509) has been confirmed by Immunocytochemistry testing in ACTA2 Knockout HeLa cells.

Other related products
We have a range of other formats of antibody clone [EPR5368] also available for your convenience: ab124964, Alexa Fluor® 488 - ab202295, Alexa Fluor® 647 - ab202296, Alexa Fluor® 555 - ab202509, Alexa Fluor® 594 - ab202510, PE - ab208844, Carrier free - ab220795, APC - ab225143, ab284414, Alexa Fluor® 790 - ab309335, ab320058, Carrier free - ab320059, ab321866, Carrier free - ab322917

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7262 PubMed40769972

2025

Plasmodium falciparum egress disrupts endothelial junctions and activates JAK-STAT signaling in a microvascular 3D blood-brain barrier model.

Applications

Unspecified application

Species

Unspecified reactive species

Livia Piatti,Alina Batzilla,Fumio Nakaki,Hannah Fleckenstein,François Korbmacher,Rory K M Long,Daniel Schraivogel,John A Hawkins,Tais Romero-Uruñuela,Borja López-Gutiérrez,Silvia Sanz Sender,Yannick Schwab,Lars M Steinmetz,James Sharpe,Maria Bernabeu

Bioengineering & translational medicine 10:e10741 PubMed40385549

2025

Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor-on-a-chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Christian R Moya-Garcia,Meghana Munipalle,Alain Pacis,Nader Sadeghi,Maryam Tabrizian,Nicole Y K Li-Jessen

International journal of molecular sciences 26: PubMed40244230

2025

Aldosterone-Induced Transformation of Vascular Smooth Muscle Cells into Macrophage-like Cells Participates in Inflammatory Vascular Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Boya Zhang,Ziqian Liu,Yi Chang,Ruyan Lv,Haixia Guo,Panpan Qiang,Tatsuo Shimosawa,Qingyou Xu,Fan Yang

Frontiers in cellular and infection microbiology 15:1543186 PubMed40061451

2025

utilizes vimentin to internalize human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoungok Jang,Hangeun Kim,Dobin Choi,Soojin Jang,Dae-Kyun Chung

Scientific reports 14:9976 PubMed38693148

2024

Eplerenone reduces lymphangiogenesis in the contralateral kidneys of UUO rats.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Hao,Panpan Qiang,Lili Fan,Yunzhao Xiong,Yi Chang,Fan Yang,Xiangting Wang,Tatsuo Shimosawa,Shengyu Mu,Qingyou Xu

Cell 186:5910-5924.e17 PubMed38070509

2023

A tridimensional atlas of the developing human head.

Applications

IHC - Wmt

Species

Human

Raphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain Chédotal

International journal of molecular sciences 24: PubMed37511521

2023

Esaxerenone Inhibits Renal Angiogenesis and Endothelial-Mesenchymal Transition via the VEGFA and TGF-β1 Pathways in Aldosterone-Infused Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Gao,Jingyue Chang,Yi Chang,Lili Fan,Ziqian Liu,Cuijuan Zhang,Tatsuo Shimosawa,Fan Yang,Qingyou Xu

Cells 12: PubMed37371070

2023

Generation of a Syngeneic Heterozygous Knockout iPS Cell Line for the In Vitro Study of HHT2-Associated Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xiang-Tischhauser,Michael Bette,Johanna R Rusche,Katrin Roth,Norio Kasahara,Boris A Stuck,Udo Bakowsky,Maria Wartenberg,Heinrich Sauer,Urban W Geisthoff,Robert Mandic

Cancer cell 41:871-886.e10 PubMed37059105

2023

Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgio Gaglia,Megan L Burger,Cecily C Ritch,Danae Rammos,Yang Dai,Grace E Crossland,Sara Z Tavana,Simon Warchol,Alex M Jaeger,Santiago Naranjo,Shannon Coy,Ajit J Nirmal,Robert Krueger,Jia-Ren Lin,Hanspeter Pfister,Peter K Sorger,Tyler Jacks,Sandro Santagata

Biology of reproduction 108:837-848 PubMed36780129

2023

Reproductive toxicity of emerging plasticizers, flame retardants, and bisphenols, using culture of the rat fetal testis†.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Tardif,Arlette Rwigemera,Natasha Letourneau,Bernard Robaire,Geraldine Delbes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368]

Immunocytochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise