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Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 555 conjugated antibody (ab202509) rabbit monoclonal antibody that is used to detect alpha smooth muscle Actin in ICC/IF, mIHC. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with alpha smooth muscle Actin knockout cell line validation


Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509), expandable thumbnail
  • Immunocytochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509), expandable thumbnail
  • Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202509), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCICC/IF
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

This product gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
1/200
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

6 products for Alternative Version

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Recommended products

Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 555 conjugated antibody (ab202509) rabbit monoclonal antibody that is used to detect alpha smooth muscle Actin in ICC/IF, mIHC. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with alpha smooth muscle Actin knockout cell line validation

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5368
Purification technique
Affinity purification Protein A
Dissociation constant
2.2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

What is this antibody validated in?


Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

Trusted by the scientific community


Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 555 (ab202509) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists


Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 555 (ab202509) has over 5 independent reviews from customers.

Related products


Antibody clone EPR5368 is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha smooth muscle Actin Alexa Fluor® 555 [EPR5368] (ab202509).

Specificity confirmed


The specificity of Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509) has been confirmed by Immunocytochemistry testing in ACTA2 Knockout HeLa cells.



Other related products


We have a range of other formats of antibody clone [EPR5368] also available for your convenience:
Anti-alpha smooth muscle Actin antibody [EPR5368] ab124964, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202295, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202296, Alexa Fluor® 555 - ab202509, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202510, PE - PE Anti-alpha smooth muscle Actin antibody [EPR5368] ab208844, Carrier free - Anti-alpha smooth muscle Actin antibody [EPR5368] - BSA and Azide free ab220795, APC - APC Anti-alpha smooth muscle Actin antibody [EPR5368] ab225143, ab284414, Alexa Fluor® 790 - ab309335, Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) ab320058, Carrier free - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free ab320059, Anti-alpha smooth muscle Actin [EPR5368] – Chicken IgY (Chimeric) ab321866, Carrier free - Anti-alpha smooth muscle Actin antibody [EPR5368] – Chicken IgY (Chimeric) – BSA and Azide Free ab322917



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Associated diseases and disorders

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509)

    ab202509 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202509 at 1/200 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509), expandable thumbnail

    Immunocytochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509)

    Alpha smooth muscle Actin Immunocytochemistry staining using rabbit Anti-alpha smooth muscle Actin antibody

    ab202509 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202509 at at 5µg/ml (shown in magenta) and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in green) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509), expandable thumbnail

    Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509)

    Immunofluorescence analysis of human lung adenocarcinoma labelled with ab202509. Image was acquired using Cell DIVE Multiplexed Imaging Solution (Leica Microsystems).

    Formalin fixed paraffin embedded (FFPE) sections were incubated with ab202509 at 1/100 dilution, for 1h at room temperature. Nuclear DNA was labelled with DAPI (Red). Deparaffinization, antigen retrieval, biomarker labeling and imaging was performed following Cell DIVE recommended standard protocol. Cell DIVE automatically processed the image to perform autofluorescence removal, distortion correction, blank glass subtraction, flat-field correction, and stitching.

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Product protocols

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