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AB311891

Alexa Fluor® 555 Anti-APC antibody [EP701Y]

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Rabbit Recombinant Monoclonal APC antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

DP2.5, APC, Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 2.5

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP701Y

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is predicted to detect isoform 2 (short) of APC based on sequence analysis.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APC or Adenomatous Polyposis Coli is a multifunctional protein involved in several cellular processes. Its molecular weight is approximately 310 kDa. The APC protein is expressed in various tissues with higher concentrations found particularly in the colon and intestinal epithelial cells. Mechanically APC acts as a scaffold protein interacting with components of the cytoskeleton and regulating processes such as cell migration and adhesion. Its ability to bind to microtubules is key in its role in stabilizing them which affects cellular polarity and division.
Biological function summary

APC plays a significant role in the regulation of the Wnt signaling pathway a critical mediator of cell growth and differentiation. APC forms part of a larger destruction complex that includes proteins like Axin and GSK-3β. This complex degrades β-catenin preventing its accumulation in the cell nucleus and therefore modulating gene transcription involved in cell proliferation. The ability of APC to mediate this process is important for maintaining proper cellular homeostasis and preventing uncontrolled cell growth that may lead to neoplasms.

Pathways

APC is integral to the Wnt signaling and PI3K/Akt pathways. The Wnt pathway primarily concerns the regulation of cellular growth with APC facilitating the degradation of β-catenin therefore inhibiting the transcription of proliferative genes. Meanwhile in the PI3K/Akt pathway interactions between APC and β-catenin may influence cell survival and apoptosis. This connectivity to PI3K and Wnt pathways highlights APC's role in both promoting and inhibiting cell division processes depending on cellular context and signaling input.

APC is intrinsically linked to colorectal cancer and familial adenomatous polyposis (FAP). Mutations or loss of function in the APC gene lead to unregulated β-catenin levels promoting tumorigenesis in the colon. In colorectal cancer the absence or dysfunction of APC disrupts normal central pathways resulting in proliferative advantage to mutated cells. Connections to β-catenin further emphasize the mechanistic role APC plays in predisposing individuals to these diseases where abnormal Wnt pathway signaling becomes deleterious. Additionally disruption in APC function and the related pathways can alter interactions with other proteins like Axin and GSK-3β which are often involved in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton (PubMed : 17293347). Plays a role in mediating the organization of F-actin into ordered bundles (PubMed : 17293347). Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules (By similarity). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
See full target information APC
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