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AB307863

Alexa Fluor® 555 Anti-Aquaporin 5 antibody [EPR3747]

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Rabbit Recombinant Monoclonal Aquaporin 5 antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

Aquaporin-5, AQP-5, AQP5

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Aquaporin 5 antibody [EPR3747] (AB307863)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Aquaporin 5 antibody [EPR3747] (AB307863)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Aquaporin 5 with ab307863 at 1/100 dilution (5.0 ug/ml). Positive staining on human colon. The section was incubated with ab307863 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Aquaporin 5 antibody [EPR3747] (AB307863)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Aquaporin 5 antibody [EPR3747] (AB307863)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Aquaporin 5 with ab307863 at 1/100 dilution (5.0 ug/ml). Negative control : no staining on human skeletal muscle. The section was incubated with ab307863 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3747

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Aquaporin 5 often abbreviated as AQP5 is a member of the aquaporin family which are integral membrane proteins that function as channels for water. This particular protein facilitates water transport across cell membranes an action critical in maintaining fluid balance. Aquaporin 5 has a molecular mass of approximately 29 kDa. It is largely expressed in epithelial cells in various tissues including the salivary glands lacrimal glands and respiratory tissues. It is recognized by different names such as Aquaporin-5 and sometimes referenced in studies as Aquazol 5.
Biological function summary

Water transport and secretion processes heavily rely on the presence of Aquaporin 5. It is integral in the formation of tight junctions in epithelial cells enhancing cell permeability to water. Unlike some other aquaporins Aquaporin 5 operates independently and is not known to be part of a larger protein complex. Its presence influences the efficiency of fluid secretion affecting activities such as salivation and tear secretion. The capacity of AQP5 to control water movement tightly connects it to cellular hydration homeostasis.

Pathways

Aquaporin 5 is significantly involved in the regulation of water balance and osmotic equilibrium in epithelial tissues. Its role in fluid secretion pathways is interconnected with other proteins like Aquaporin 4 which share similar duties in the osmotic control of water. In the water transport pathways AQP5 coordinates with proteins like CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) to manage fluid movement particularly in the respiratory passages and glands. The precise regulation of water flow by these pathways ensures that overall tissue hydration remains constant.

Aquaporin 5 is directly associated with conditions such as Sjögren's syndrome and xerostomia (dry mouth syndrome). During Sjögren's syndrome malfunction of AQP5 can lead to impaired salivary and tear secretion contributing to the symptoms of dryness. Furthermore defects or polymorphisms in AQP5 have implications in respiratory diseases potentially affecting proteins like Aquaporin 3 which shares functional similarities. Understanding the role of AQP5 in these conditions enables further exploration into therapeutic targets and treatment methods.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (PubMed : 18768791, PubMed : 8621489). Plays an important role in fluid secretion in salivary glands (By similarity). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed : 16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity).
See full target information AQP5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB239904

Anti-Aquaporin 5 antibody [EPR3747] - BSA and Azide free

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aquaporin-5-antibody-epr3747-bsa-and-azide-free-ab239904

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Alternative Version
Primary Antibodies

AB308598

Alexa Fluor® 488 Anti-Aquaporin 5 antibody [EPR3747]

primary-antibodies

alexa-fluor-488-aquaporin-5-antibody-epr3747-ab308598

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com