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AB320124

Alexa Fluor® 555 Anti-ARPC5/p16 ARC antibody [EP1551Y]

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Rabbit Recombinant Monoclonal ARPC5/p16 ARC antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

ARC16, ARPC5, Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 578 PE

    PE Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 660 APC

    APC Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • Unconjugated

    Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • Carrier free

    Anti-ARPC5/p16 ARC antibody [EP1551Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1551Y

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to the p16 ARC protein O15511. There is no observed cross reactivity to the CDKN2A/p16INK4a protein.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARPC5 also known as p16 ARC is a protein with a molecular weight of roughly 16 kilodaltons. It localizes largely in the cytoplasm and expresses in many cell types across species. The ARPC5 protein is a subunit of the Arp2/3 complex a vital component responsible for actin nucleation and branching during cytoskeleton remodeling. Researchers often examine ARPC5 through techniques such as western blot and immunohistochemistry using specific antibodies like anti-p16 or ARPC5 anticuerpo to identify its presence and distribution.
Biological function summary

The ARPC5 protein is essential for effective actin polymerization making it an important contributor to cell motility and structure. As part of the Arp2/3 complex it interacts with other proteins to stimulate the growth of new actin filaments. This activity is important in processes like endocytosis phagocytosis and cell migration. Furthermore ARPC5 plays a role in maintaining cellular homeostasis and is involved in cellular signaling pathways.

Pathways

Arpc5 influences the regulation of the actin cytoskeleton which intersects with pathways associated with cell movement and morphogenesis. It prominently relates to the WAVE complex working synergistically to regulate actin dynamics. ARPC5's involvement in the Rac1-WAVE-Arp2/3 pathway highlights its relationship with Rac1 a small GTPase known for regulating the cytoskeleton and cellular functions like adhesion and migration.

Imbalances in ARPC5 function can link to cancer progression and neurodegenerative diseases. Aberrant activity of the Arp2/3 complex which involves ARPC5 associates with increased cell invasion and metastasis in cancer. Additionally disruptions in ARPC5 activity connect to neurodegenerative conditions where cytoskeletal arrangement alterations play a role. The protein's interaction with others in the complex such as Rac1 further deepens its involvement in these diseases signaling potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ARPC5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com