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AB312055

Alexa Fluor® 555 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

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Rabbit Recombinant Monoclonal FABP-1 antibody - conjugated to Alexa Fluor® 555. Suitable for Target Binding Affinity, Antibody Labelling, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm, cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • HRP

    HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 578 PE

    PE Anti-Aspartate Aminotransferase +FABP-1 antibody [EPR12145]

  • 660 APC

    APC Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • Unconjugated

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • Carrier free

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12145

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

Antibody Labelling, ICC/IF, IHC-P, Target Binding Affinity, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase also known as AST or alkaline aminotransferase is an important enzyme in amino acid metabolism. It catalyzes the transfer of an amino group from aspartate to alpha-ketoglutarate forming glutamate and oxaloacetate. This enzyme has an approximate molecular mass of 46 kDa and is expressed in the liver heart skeletal muscle and kidney tissues. AST plays a significant role in the intracellular balance of amino acids and is an important biomarker in clinical diagnostics.
Biological function summary

AST functions in the transamination process a mechanism central to the synthesis and degradation of amino acids. This process is fundamental in the production of energy and the maintenance of the urea cycle. AST does not form a part of a complex but interacts dynamically with substrates and cofactors like pyridoxal phosphate essential for its enzymatic activity. The activity of AST is important in ensuring the proper function of amino acid metabolism in various tissues.

Pathways

AST operates within the citric acid cycle and the urea cycle. In the citric acid cycle AST contributes to the interconversion of amino acids and metabolic intermediates aiding in energy production. It links to proteins such as malate dehydrogenase and glutamate dehydrogenase which play roles in associated metabolic conversions and energy cycles. These interactions reflect AST's integration in maintaining cellular metabolic processes.

AST levels serve as an indicator of liver health and are often elevated in conditions such as hepatitis and myocardial infarction. Hepatitis causes liver damage resulting in increased AST release into the bloodstream. In myocardial infarction damaged cardiac tissue releases AST providing a diagnostic cue for tissue injury. Elevated AST levels can associate with other enzymes like alanine aminotransferase (ALT) used together to assess liver and heart function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
See full target information GOT2

Additional targets

Aspartate aminotransferase, cytoplasmic
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com