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AB210732

Alexa Fluor® 555 Anti-ATP synthase C antibody [EPR13907]

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(1 Publication)

Rabbit Recombinant Monoclonal ATP synthase C antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ATP5G1, ATP5MC1, ATP synthase lipid-binding protein, ATP synthase membrane subunit c locus 1, ATP synthase proteolipid P1, ATP synthase proton-transporting mitochondrial F(0) complex subunit C1, ATPase protein 9, ATPase subunit c

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-ATP synthase C antibody [EPR13907] (AB210732)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-ATP synthase C antibody [EPR13907] (AB210732)

ab210732 staining ATP synthase C in HepG2 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210732 at 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ATP synthase C antibody [EPR13907]

  • Unconjugated

    Anti-ATP synthase C antibody [EPR13907]

  • Carrier free

    Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13907

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP synthase subunit c also known as ATP synthase C ATP-C or ATP synthase C subunit is an integral component of the ATP synthase complex. This protein has a known molecular mass of approximately 8.3 kDa. It is prominently expressed in mitochondrial membranes where it serves as an important part of the proton channel. Its primary function involves facilitating the conversion of proton motive force into rotational mechanical energy which drives ATP synthesis. Through this mechanical action ATP synthase C contributes significantly to energy conversion within cells.
Biological function summary

ATP synthase C functions as a critical component of the ATP synthase enzyme complex also known as FoF1 ATPase or Complex V of the oxidative phosphorylation pathway. This subunit forms a ring structure that allows protons to move across the mitochondrial membrane which is necessary for the synthesis of ATP from ADP and inorganic phosphate. By participating in this synthesis process ATP synthase C impacts cellular energy metabolism affecting various physiological functions dependent on ATP as an energy source.

Pathways

ATP synthase C plays an essential role within the oxidative phosphorylation pathway. This pathway is integral to cellular respiration and is responsible for the majority of ATP production in aerobic organisms. ATP synthase C interacts with other proteins such as the ATP synthase alpha and beta subunits which form the catalytic core of the enzyme. These interactions are central to the energy currency of the cell ensuring efficient energy conversion and transfer.

ATP synthase C is implicated in mitochondrial dysfunctions and associated conditions such as mitochondrial myopathies and neurodegenerative diseases. Mitochondrial myopathies result from defects in the oxidative phosphorylation pathway often involving proteins like cytochrome c oxidase which are important for efficient ATP production. Disorders related to ATP synthase C dysfunction can cause significant impacts on tissues that require high energy reflecting its critical role in maintaining cellular energy balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
See full target information ATP5MC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virology 573:50-58 PubMed35714458

2022

Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency.

Applications

Unspecified application

Species

Unspecified reactive species

Nadejda Beliakova-Bethell,Antigoni Manousopoulou,Savitha Deshmukh,Amey Mukim,Douglas D Richman,Spiros D Garbis,Celsa A Spina
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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