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AB314337

Alexa Fluor® 555 Anti-C5a-R antibody [EPR23278-117]

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Rabbit Recombinant Monoclonal C5a-R antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

CD88, C5AR, C5R1, C5AR1, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-C5a-R antibody [EPR23278-117] (AB314337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-C5a-R antibody [EPR23278-117] (AB314337)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling C5a-R with ab314337 at 1/50 (10.0 ug/ml). Membranous staining on human tonsil. The section was incubated with ab314337 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-C5a-R antibody [EPR23278-117] (AB314337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-C5a-R antibody [EPR23278-117] (AB314337)

Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue labeling C5a-R with ab314337 at 1/50 (10.0 ug/ml). Membranous staining on human kidney cancer. The section was incubated with ab314337 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-C5a-R antibody [EPR23278-117]

  • 578 PE

    PE Anti-C5a-R antibody [EPR23278-117]

  • 660 APC

    APC Anti-C5a-R antibody [EPR23278-117]

  • Unconjugated

    Anti-C5a-R antibody [EPR23278-117]

  • Carrier free

    Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23278-117

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5a-R also known as CD88 or C5aR1 is a protein found primarily on the surface of immune cells like macrophages and neutrophils. Its mass is approximately 45 kDa. C5a-R functions as a G protein-coupled receptor (GPCR) and binds the complement component C5a a powerful inflammatory peptide. The receptor is expressed in various tissues but primarily in the immune system where it plays a significant role in immune responses and inflammatory processes.
Biological function summary

C5a-R functions as a mediator of inflammation and immune responses. It is a part of the complement system a complex of proteins involved in innate immunity. Upon binding to C5a this receptor initiates a signaling cascade that leads to the recruitment and activation of immune cells at sites of inflammation. It amplifies the inflammatory response leading to cell chemotaxis degranulation and the production of pro-inflammatory cytokines.

Pathways

C5a-R plays an important role in the complement and immune pathways. It interacts closely with the C5 protein which is a core component of the complement pathway. This receptor also connects to other immune system proteins such as the C3 and C4 proteins through the cascade. Together these interactions help defend against pathogenic infections and maintain immune homeostasis.

C5a-R is associated with conditions like rheumatoid arthritis and sepsis. In rheumatoid arthritis the receptor's activation by C5a contributes to chronic inflammation and joint damage. During sepsis overactivation of C5a-R can lead to excessive inflammation and tissue damage. The receptor's involvement in these diseases is often linked with other proteins in the complement system such as C3a and C4a which also play roles in modulating inflammation and immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed : 10636859, PubMed : 15153520, PubMed : 1847994, PubMed : 29300009, PubMed : 7622471, PubMed : 8182049, PubMed : 9553099). The ligand interacts with at least two sites on the receptor : a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed : 7622471, PubMed : 8182049, PubMed : 9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed : 10636859, PubMed : 15153520).
See full target information C5AR1
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Product promise

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For full details, please see our Terms & Conditions

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