JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB280849

Alexa Fluor® 555 Anti-CD4 antibody [EPR6855]

5

(2 Reviews)

|

(3 Publications)

Anti-CD4 antibody [EPR6855] - Alexa Fluor® 555 conjugated (ab280849) is a rabbit recombinant monoclonal antibody detecting CD4 in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD4 antibody [EPR6855] (AB280849)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD4 antibody [EPR6855] (AB280849)

Immunofluorescence staining of CD4 in a section of formalin-fixed paraffin-embedded human normal tonsil*.

Performed on a Leica BOND®. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 20 minutes. The section was then incubated at room temperature for 1 hour with ab280849 at 1/100 dilution (shown in green) and counterstained using ab190573, Rabbit anti-Tubulin (A647)(1/250) at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6855

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
style
left-column

Product details

What is this antibody validated in?
Alexa Fluor® 555 Anti-CD4 antibody [EPR6855] (ab280849) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR6855 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD4 Alexa Fluor® 555 [EPR6855] (ab280849).

Other related products
We have a range of other formats of antibody clone [EPR6855] also available for your convenience: ab133616, Carrier free - ab181724, HRP - ab195842, Alexa Fluor® 647 - ab196147, Alexa Fluor® 488 - ab196372, Alexa Fluor® 594 - ab277931, Alexa Fluor® 555 - ab280849, ab284581, Carrier free - ab307492, ab317787, Carrier free - ab317797

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1475146 PubMed39497830

2024

Lesional senescent CD4 T cells mediate bystander cytolysis and contribute to the skin pathology of human cutaneous leishmaniasis.

Applications

Unspecified application

Species

Unspecified reactive species

Luciana Polaco Covre,Carlos Henrique Fantecelle,Renan Garcia de Moura,Paola Oliveira Lopes,Isabela Valim Sarmento,Celio Geraldo Freire-de-Lima,Debora Decote-Ricardo,Herbert Leonel de Matos Guedes,Alessandra Marcia da Fonsceca-Martins,Lucas Pedreira de Carvalho,Edgar Marcelino de Carvalho,David M Mosser,Aloisio Falqueto,Arne N Akbar,Daniel Claudio Oliveira Gomes

Nature immunology 25:2152-2165 PubMed39438660

2024

A longitudinal single-cell atlas of anti-tumour necrosis factor treatment in inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Thomas,Matthias Friedrich,Charlotte Rich-Griffin,Mathilde Pohin,Devika Agarwal,Julia Pakpoor,Carl Lee,Ruchi Tandon,Aniko Rendek,Dominik Aschenbrenner,Ashwin Jainarayanan,Alexandru Voda,Jacqueline H Y Siu,Raphael Sanches-Peres,Eloise Nee,Dharshan Sathananthan,Dylan Kotliar,Peter Todd,Maria Kiourlappou,Lisa Gartner,Nicholas Ilott,Fadi Issa,Joanna Hester,Jason Turner,Saba Nayar,Jonas Mackerodt,Fan Zhang,Anna Jonsson,Michael Brenner,Soumya Raychaudhuri,Ruth Kulicke,Danielle Ramsdell,Nicolas Stransky,Ray Pagliarini,Piotr Bielecki,Noah Spies,Brian Marsden,Stephen Taylor,Allon Wagner,Paul Klenerman,Alissa Walsh,Mark Coles,Luke Jostins-Dean,Fiona M Powrie,Andrew Filer,Simon Travis,Holm H Uhlig,Calliope A Dendrou,Christopher D Buckley

Nature immunology 23:1256-1272 PubMed35902638

2022

Partial RAG deficiency in humans induces dysregulated peripheral lymphocyte development and humoral tolerance defect with accumulation of T-bet B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztian Csomos,Boglarka Ujhazi,Peter Blazso,Jose L Herrera,Christopher M Tipton,Tomoki Kawai,Sumai Gordon,Maryssa Ellison,Kevin Wu,Matthew Stowell,Lauren Haynes,Rachel Cruz,Bence Zakota,Johnny Nguyen,Michelle Altrich,Christoph B Geier,Svetlana Sharapova,Joseph F Dasso,Jennifer W Leiding,Grace Smith,Waleed Al-Herz,Mayra de Barros Dorna,Olajumoke Fadugba,Eva Fronkova,Veronika Kanderova,Michael Svaton,Sarah E Henrickson,Joseph D Hernandez,Taco Kuijpers,Snezhina Mihailova Kandilarova,Elizaveta Naumova,Tomas Milota,Anna Sediva,Despina Moshous,Benedicte Neven,Tara Saco,Ravishankar Sargur,Sinisa Savic,John Sleasman,Gauri Sunkersett,Brant R Ward,Masanobu Komatsu,Stefania Pittaluga,Attila Kumanovics,Manish J Butte,Michael P Cancro,Shiv Pillai,Eric Meffre,Luigi D Notarangelo,Jolan E Walter
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB307335

Alexa Fluor® 555 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD3 antibody [SP162] (AB307335)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB199397

PE Anti-ZNFN1A2/HELIOS antibody [22F6]

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com