Alexa Fluor® 555 Anti-CD68 antibody [KP1]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD68 antibody [KP1] (AB279323)

Immunofluorescence staining of CD68 staining in a section of formalin-fixed paraffin-embedded human normal tonsil*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 20 minutes. The section was then incubated at room temperature for 1 hour with ab279323 at 1/100 dilution (shown in red) and counterstained using ab195887, Ms anti-Tubulin (A488) (1/250) at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD68 antibody [KP1] (AB279323)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD68 with ab282006 at 1/100 dilution (5.0 μg/ml).

Positive staining on macrophages in the human tonsil. The section was then incubated overnight at +4 °C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab279323 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount ^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD68 antibody [KP1] (AB279323)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD68 with ab282006 at 1/100 dilution (5.0 μg/ml).

Positive staining on Kuffer cells in the human liver. The section was then incubated overnight at +4 °C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab279323 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount ^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD68 antibody [KP1] (AB279323)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling CD68 with ab282006 at 1/100 dilution (5.0 μg/ml).

Negative control : No staining on human skeletal muscle. The section was then incubated overnight at +4 °C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab279323 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount ^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)