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AB217238

Alexa Fluor® 555 Anti-CHD1L antibody [EPR14515(2)]

5

(4 Reviews)

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(1 Publication)

Rabbit Recombinant Monoclonal CHD1L antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ALC1, CHD1L, Chromodomain-helicase-DNA-binding protein 1-like, Amplified in liver cancer protein 1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CHD1L antibody [EPR14515(2)] (AB217238)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CHD1L antibody [EPR14515(2)] (AB217238)

ab217238 staining CHD1L in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab217238 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14515(2)

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CHD1L gene encodes a protein known as chromodomain helicase DNA-binding protein 1-like. This protein plays a role in chromatin remodeling using the energy from ATP hydrolysis to alter chromatin structure thereby facilitating processes such as DNA repair and transcription. The mass of CHD1L is approximately 120 kDa. This protein is expressed in various tissues with a notable presence in liver and cancerous tissues indicating its potential involvement in cellular proliferation and repair mechanisms.
Biological function summary

The function of CHD1L is significant in genomic stability and response to DNA damage. It acts as a part of the chromatin remodeling complex and contributes to the regulation of chromatin dynamics affecting gene expression and repair pathways. CHD1L interacts with other proteins involved in DNA repair ensuring the cell maintains genetic integrity under stress conditions.

Pathways

CHD1L is involved in the DNA damage response and chromatin organization pathways. It interacts with RAD51-associated protein which is important for homologous recombination repair and collaborates with proteins in the pathway to restore DNA integrity after damage. CHD1L's activity in these pathways highlights its role in maintaining cellular homeostasis and preventing genomic instability.

CHD1L has been implicated in hepatocellular carcinoma and ovarian cancer. The overexpression of CHD1L in such cancers suggests a role in cancer progression by promoting cell proliferation and survival. Additionally its interaction with HNF4A a protein involved in liver cancer accentuates its impact on oncogenic pathways. Understanding CHD1L in these contexts could offer insights into therapeutic targets for cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent chromatin remodeler that mediates chromatin-remodeling following DNA damage (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653, PubMed : 33357431, PubMed : 34210977, PubMed : 34486521, PubMed : 34874266). Recruited to DNA damage sites through interaction with poly-ADP-ribose : specifically recognizes and binds histones that are poly-ADP-ribosylated on serine residues in response to DNA damage (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653, PubMed : 34486521, PubMed : 34874266). Poly-ADP-ribose-binding activates the ATP-dependent chromatin remodeler activity, thereby regulating chromatin during DNA repair (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653, PubMed : 34486521, PubMed : 34874266). Catalyzes nucleosome sliding away from DNA breaks in an ATP-dependent manner (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653). Chromatin remodeling activity promotes PARP2 removal from chromatin (PubMed : 33275888).
See full target information CHD1L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 10:1178439 PubMed37426420

2023

Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph P Clarke,Patricia A Thibault,Sakina Fatima,Hannah E Salapa,Subha Kalyaanamoorthy,Aravindhan Ganesan,Michael C Levin
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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