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AB312154

Alexa Fluor® 555 Anti-CHD3 antibody [EPNCIR110A]

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Rabbit Recombinant Monoclonal CHD3 antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

2600010P09Rik, AF020312, ATP-dependent helicase Chd3, CHD3_HUMAN, Chromodomain-helicase-DNA-binding protein 3, MGC40857, Mi 2a, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Prp7, Prp9 1, ZFH, Zinc finger helicase, hZFH, zinc-finger helicase (Snf2-like)

  • 578 PE

    PE Anti-CHD3 antibody [EPNCIR110A]

  • 660 APC

    APC Anti-CHD3 antibody [EPNCIR110A]

  • HRP

    HRP Anti-CHD3 antibody [EPNCIR110A]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CHD3 antibody [EPNCIR110A]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CHD3 antibody [EPNCIR110A]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CHD3 antibody [EPNCIR110A]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CHD3 antibody [EPNCIR110A]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CHD3 antibody [EPNCIR110A]

  • Unconjugated

    Anti-CHD3 antibody [EPNCIR110A]

  • Carrier free

    Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR110A

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHD3 also known as Chromodomain Helicase DNA Binding Protein 3 is a protein involved in transcription regulation. The protein has a molecular mass of approximately 220 kDa. CHD3 is a member of the chromatin remodeler family and is expressed widely throughout various tissues. It contains dual chromodomains a helicase/ATPase domain and a DNA-binding domain enabling it to remodel chromatin structure by altering nucleosome positioning on DNA.
Biological function summary

CHD3 plays a significant role in modifying chromatin architecture and regulating the accessibility of the DNA to transcription factors. It functions as part of the NuRD (Nucleosome Remodeling and Deacetylase) complex which combines the features of ATP-dependent chromatin remodeling and histone deacetylase activity. CHD3 helps remodel nucleosomes and remove acetyl groups from histones which leads to chromatin condensation and transcriptional repression. Its activity influences genes involved in development differentiation and cell cycle control.

Pathways

CHD3's function integrates into pathways that control gene expression and DNA repair. It plays a role in the transcriptional regulation pathway working alongside proteins like HDAC1 and NuRD components. Additionally CHD3 participates in the DNA damage response cooperating with proteins such as CHD4 and RBBP4 to ensure genomic stability. These interactions and functions place CHD3 at a strategic position within cellular mechanisms maintaining transcription fidelity and genomic integrity.

CHD3 associations with cancer and developmental disorders stand out. Abnormal expression or mutations in CHD3 can contribute to tumorigenesis as it can affect the expression and repression of oncogenes and tumor suppressor genes. Furthermore CHD3 mutations have been linked to Snijders Blok-Campeau syndrome a neurodevelopmental disorder. The interaction between CHD3 and other proteins like HDAC1 and CHD4 plays a critical role in the manifestation of these conditions influencing pathways that drive disease progression and phenotype expression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CHD3
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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