AB203444

Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y]

Name: Alexa Fluor® 555 conjugated Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444) | Abcam
Brand: Abcam Limited
SKU: AB203444
Price: 680 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(1 Publication)

Anti-Cytokeratin 19 antibody [EP1580Y] - Alexa Fluor® 555 conjugated (ab203444) is a rabbit recombinant monoclonal antibody detecting Cytokeratin 19 in ICC/IF. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203444)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203444)

ab203444 staining Cytokeratin 19 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203444 at 1/200 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203444)

ab203444 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab203444 at 1/500 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Unconjugated

Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y]
578 PE

PE Anti-Cytokeratin 19 antibody [EP1580Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]
660 APC

APC Anti-Cytokeratin 19 antibody [EP1580Y]
Carrier free

Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free
HRP

HRP Anti-Cytokeratin 19 antibody [EP1580Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1580Y

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP1580Y is also available pre-conjugated to a variety of labels for your convenience – Anti-Cytokeratin 19 Alexa Fluor® 555 [EP1580Y] (ab203444).

Specificity confirmed
The specificity of Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444) has been confirmed by Immunocytochemistry testing in Cytokeratin 19 Knockout MCF7 cells.

Other related products
We have a range of other formats of antibody clone [EP1580Y] also available for your convenience: ab52625, Alexa Fluor® 488 - ab192643, Alexa Fluor® 647 - ab192980, HRP - ab193600, Carrier free - ab195872, Alexa Fluor® 594 - ab203443, Alexa Fluor® 555 - ab203444, Alexa Fluor® 568 - ab203445, APC - ab224980, PE - ab224981, Carrier free - ab302465, ab323561, ab323562

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT19

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 57:1400-1420.e7 PubMed35617956

2022

A human breast atlas integrating single-cell proteomics and transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

G Kenneth Gray,Carman Man-Chung Li,Jennifer M Rosenbluth,Laura M Selfors,Nomeda Girnius,Jia-Ren Lin,Ron C J Schackmann,Walter L Goh,Kaitlin Moore,Hana K Shapiro,Shaolin Mei,Kurt D'Andrea,Katherine L Nathanson,Peter K Sorger,Sandro Santagata,Aviv Regev,Judy E Garber,Deborah A Dillon,Joan S Brugge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203444)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203444)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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