AB203434

Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody - conjugated to Alexa Fluor® 555. Cytoskeleton marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB203434)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB203434)

ab203434 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203434 at 1/100 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in T47D cells fixed with 100% methanol (5 min).

578 PE

PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
Unconjugated

Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1619Y

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Human, Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

See full target information KRT7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6420 PubMed40645983

2025

Forced expression of MSR repeat transcripts above a threshold limit breaks heterochromatin organisation.

Applications

Unspecified application

Species

Unspecified reactive species

Reagan W Ching,Kalina M Świst-Rosowska,Galina Erikson,Birgit Koschorz,Bettina Engist,Thomas Jenuwein

Nature communications 13:943 PubMed35177608

2022

Spatiotemporal dynamics of clonal selection and diversification in normal endometrial epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Manako Yamaguchi,Hirofumi Nakaoka,Kazuaki Suda,Kosuke Yoshihara,Tatsuya Ishiguro,Nozomi Yachida,Kyota Saito,Haruka Ueda,Kentaro Sugino,Yutaro Mori,Kaoru Yamawaki,Ryo Tamura,Sundaramoorthy Revathidevi,Teiichi Motoyama,Kazuki Tainaka,Roel G W Verhaak,Ituro Inoue,Takayuki Enomoto

Cell reports. Medicine 3:100525 PubMed35243422

2022

An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Brett E Johnson,Allison L Creason,Jayne M Stommel,Jamie M Keck,Swapnil Parmar,Courtney B Betts,Aurora Blucher,Christopher Boniface,Elmar Bucher,Erik Burlingame,Todd Camp,Koei Chin,Jennifer Eng,Joseph Estabrook,Heidi S Feiler,Michael B Heskett,Zhi Hu,Annette Kolodzie,Ben L Kong,Marilyne Labrie,Jinho Lee,Patrick Leyshock,Souraya Mitri,Janice Patterson,Jessica L Riesterer,Shamilene Sivagnanam,Julia Somers,Damir Sudar,Guillaume Thibault,Benjamin R Weeder,Christina Zheng,Xiaolin Nan,Reid F Thompson,Laura M Heiser,Paul T Spellman,George Thomas,Emek Demir,Young Hwan Chang,Lisa M Coussens,Alexander R Guimaraes,Christopher Corless,Jeremy Goecks,Raymond Bergan,Zahi Mitri,Gordon B Mills,Joe W Gray

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB203434)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise