AB209601

Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR17078]

Name: Anti-Cytokeratin 7 Alexa Fluor® 555 antibody [EPR17078] (ab209601) | Abcam
Brand: Abcam Limited
SKU: AB209601
Price: 630 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(10 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples. Cited in 10 publications.

View Alternative Names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR17078] (AB209601)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR17078] (AB209601)

ab209601 staining Cytokeratin 7 in A549 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209601 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in A549 cells fixed with 4% formaldehyde (10 min).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR17078]
HRP

HRP Anti-Cytokeratin 7 antibody [EPR17078]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR17078]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR17078]
578 PE

PE Anti-Cytokeratin 7 antibody [EPR17078]
Unconjugated

Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17078

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in A549 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

See full target information KRT7

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4578 PubMed38811586

2024

Mucus production, host-microbiome interactions, hormone sensitivity, and innate immune responses modeled in human cervix chips.

Applications

Unspecified application

Species

Unspecified reactive species

Zohreh Izadifar,Justin Cotton,Siyu Chen,Viktor Horvath,Anna Stejskalova,Aakanksha Gulati,Nina T LoGrande,Bogdan Budnik,Sanjid Shahriar,Erin R Doherty,Yixuan Xie,Tania To,Sarah E Gilpin,Adama M Sesay,Girija Goyal,Carlito B Lebrilla,Donald E Ingber

Cell reports 42:113293 PubMed37858468

2023

Single-cell and spatial analyses reveal a tradeoff between murine mammary proliferation and lineage programs associated with endocrine cues.

Applications

Unspecified application

Species

Unspecified reactive species

G Kenneth Gray,Nomeda Girnius,Hendrik J Kuiken,Aylin Z Henstridge,Joan S Brugge

Cell genomics 2: PubMed36419822

2022

Spatial intra-tumor heterogeneity is associated with survival of lung adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Jun Wu,Daniel Temko,Zoltan Maliga,Andre L Moreira,Emi Sei,Darlan Conterno Minussi,Jamie Dean,Charlotte Lee,Qiong Xu,Guillaume Hochart,Connor A Jacobson,Clarence Yapp,Denis Schapiro,Peter K Sorger,Erin H Seeley,Nicholas Navin,Robert J Downey,Franziska Michor

Nature protocols 17:1658-1690 PubMed35546639

2022

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.

Applications

Unspecified application

Species

Unspecified reactive species

Rajendra Kumar Gurumurthy,Stefanie Koster,Naveen Kumar,Thomas F Meyer,Cindrilla Chumduri

Micromachines 13: PubMed35630139

2022

In Situ Electroporation on PERFECT Filter for High-Efficiency and High-Viability Tumor Cell Labeling.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Hun,Yi Zhang,Qingmei Xu,Dong Huang,Qi Wang,Zhihong Li,Wei Wang

Annals of translational medicine 9:1476 PubMed34734028

2021

Human menstrual blood-derived mesenchymal stem cells regulation of the EGF/Ras p21 pathway as a potential therapeutic target for thin endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Zhao,Fengli Chi,Tingyu Zhang,Xiaoming Teng,Kunming Li

Cancer cell 39:1623-1642.e20 PubMed34739845

2021

Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jaikumar Duraiswamy,Riccardo Turrini,Aspram Minasyan,David Barras,Isaac Crespo,Alizée J Grimm,Julia Casado,Raphael Genolet,Fabrizio Benedetti,Alexandre Wicky,Kalliopi Ioannidou,Wilson Castro,Christopher Neal,Amandine Moriot,Stéphanie Renaud-Tissot,Victor Anstett,Noémie Fahr,Janos L Tanyi,Monika A Eiva,Connor A Jacobson,Kathleen T Montone,Marie Christine Wulff Westergaard,Inge Marie Svane,Lana E Kandalaft,Mauro Delorenzi,Peter K Sorger,Anniina Färkkilä,Olivier Michielin,Vincent Zoete,Santiago J Carmona,Periklis G Foukas,Daniel J Powell,Sylvie Rusakiewicz,Marie-Agnès Doucey,Denarda Dangaj Laniti,George Coukos

Cancers 13: PubMed33922695

2021

A Novel Multidrug-Resistant Cell Line from an Italian Intrahepatic Cholangiocarcinoma Patient.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Peraldo-Neia,Annamaria Massa,Francesca Vita,Marco Basiricò,Chiara Raggi,Paola Bernabei,Paola Ostano,Laura Casorzo,Mara Panero,Francesco Leone,Giuliana Cavalloni,Massimo Aglietta

Nature cell biology 23:184-197 PubMed33462395

2021

Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Cindrilla Chumduri,Rajendra Kumar Gurumurthy,Hilmar Berger,Oliver Dietrich,Naveen Kumar,Stefanie Koster,Volker Brinkmann,Kirstin Hoffmann,Marina Drabkina,Panagiota Arampatzi,Dajung Son,Uwe Klemm,Hans-Joachim Mollenkopf,Hermann Herbst,Mandy Mangler,Jörg Vogel,Antoine-Emmanuel Saliba,Thomas F Meyer

eLife 7: PubMed29993362

2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Benjamin Izar,Shu Wang,Clarence Yapp,Shaolin Mei,Parin M Shah,Sandro Santagata,Peter K Sorger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR17078]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR17078] (AB209601)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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