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AB312079

Alexa Fluor® 555 Anti-EB3 antibody [EPR11421(B)]

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Rabbit Recombinant Monoclonal EB3 antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

Microtubule-associated protein RP/EB family member 3, EB1 protein family member 3, End-binding protein 3, RP3, EBF3, EB3, MAPRE3

  • Unconjugated

    Anti-EB3 antibody [EPR11421(B)]

  • 660 APC

    APC Anti-EB3 antibody [EPR11421(B)]

  • HRP

    HRP Anti-EB3 antibody [EPR11421(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-EB3 antibody [EPR11421(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-EB3 antibody [EPR11421(B)]

  • 578 PE

    PE Anti-EB3 antibody [EPR11421(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-EB3 antibody [EPR11421(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-EB3 antibody [EPR11421(B)]

  • Carrier free

    Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11421(B)

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EB3 also known as End-binding protein 3 is a microtubule-associated protein with a molecular weight of approximately 32 kDa. This protein belongs to the EB family of proteins which includes EB1 and EB2. EB3 facilitates microtubule dynamics by binding to the plus ends of microtubules and playing a role in their stabilization and elongation. Researchers have observed its expression in various tissues with a notable presence in neuronal tissues where it supports complex cellular activities.
Biological function summary

EB3 interacts with other end-binding proteins as part of the plus-end tracking complex often abbreviated as +TIPs. This complex modulates the behavior of microtubules essential for processes like mitosis and cell migration. EB3’s interaction within this complex stabilizes microtubules at the growing edge important for neuronal development and synaptic function. Additionally EB3 helps in intracellular signaling through direct interaction with other microtubule-associated proteins thereby contributing to cellular structural integrity.

Pathways

EB3 plays an integral role in the Wnt signaling pathway and the microtubule polymerization regulation pathway. These pathways are important for normal cell division and signaling. In the Wnt signaling pathway EB3 interacts with proteins like APC essential for the pathway’s downstream signaling events that influence cell polarity and differentiation. Moreover its involvement in the polymerization regulation pathway helps in facilitating effective cargo transport along microtubules.

EB3 has been linked to neurodegenerative diseases such as Alzheimer’s disease and certain types of cancer. Abnormalities in EB3 expression and function can lead to disruptions in microtubule stability contributing to the pathogenesis of these conditions. EB3's connection with tau protein further highlights its role in Alzheimer’s where tau aggregation negatively affects microtubule function. In cancer alterations in EB3 expression influence tumor progression by impacting cell motility and invasiveness with implications for metastasis development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed : 19255245, PubMed : 28814570). Promotes microtubule growth (PubMed : 19255245, PubMed : 28814570). May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed : 19255245, PubMed : 28814570). Also acts as a regulator of minus-end microtubule organization : interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed : 28814570).
See full target information MAPRE3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB246357

Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free

primary-antibodies

eb3-antibody-epr11421b-bsa-and-azide-free-ab246357

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