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AB311975

Alexa Fluor® 555 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

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Rabbit Recombinant Monoclonal eEF1A1/EF-Tu antibody - conjugated to Alexa Fluor® 555. Suitable for Target Binding Affinity, Antibody Labelling, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

EEF1A, EF1A, LENG7, EEF1A1, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EF-Tu, eEF1A-1, EEF1AL3, EEF1A1P5, Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5, eEF1A-like 3

  • 578 PE

    PE Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 660 APC

    APC Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • HRP

    HRP Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • Unconjugated

    Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • Carrier free

    Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9471

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, Target Binding Affinity, ICC/IF, Antibody Labelling, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 6 continuous identical amino acids with eEF1A2. Cross-reactivity with this protein has not been confirmed experimentally.

EE1AL3/EEF1A1P5 it's a suspected pseudogene (https://www.ncbi.nlm.nih.gov/gene/158078). Our validation shows that this clone is able to detect Recombinant EF1AL3/EEF1A1P5 but endogenous protein expression is yet unknown.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF1A1 also known as EF-Tu in prokaryotes functions as an elongation factor in the process of protein synthesis. It facilitates the delivery of aminoacyl-tRNA to the ribosome during the elongation phase of translation. eEF1A1 exhibits a mass of approximately 50 kDa and shows high expression levels in various tissues particularly in the liver and neuronal tissues. It shares functional similarities with EF-G which plays a role in translocation during translation. Moreover eEF1A1 appears relevant in cellular processes beyond translation contributing to its importance across cellular functions.
Biological function summary

EEF1A1 acts as an important component in the machinery of protein synthesis and takes part in the elongation factor complex. Within this complex eEF1A1 interacts with other molecules to ensure the accuracy and efficiency of codon-anticodon pairing on the ribosome. Additionally eEF1A1 associates with actin and participates in the cytoskeleton organization. This multifunctional currency of eEF1A1 suggests its involvement in diverse cellular processes beyond classic translation tasks.

Pathways

The role of eEF1A1 in protein synthesis positions it within the larger context of the mTOR signaling pathway important for cellular growth proliferation and metabolism. Since its function bridges translation initiation and elongation it interacts with proteins like mTOR and S6K1. Within the MAPK pathway eEF1A1 shows interactions influencing cellular survival and apoptosis. These interactions connect eEF1A1 to essential regulatory mechanisms that maintain cellular homeostasis and adaptability.

EEF1A1 exhibits significant involvement in cancer and neurodegenerative diseases. Overexpression or mutation of eEF1A1 frequently associates with various cancers implicating its role in tumor progression and cell proliferation. Interactions with proteins such as p53 link eEF1A1 to pathways that influence tumor suppression and genomic stability. In neurodegenerative conditions like Alzheimer's disease altered eEF1A1 behavior and expression affect neuronal viability and tau protein interaction suggesting its potential contribution to the pathogenesis of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed : 17177976). Also plays a role in cytoskeleton organization by promoting actin bundling (By similarity).. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.
See full target information EEF1A1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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