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AB311915

Alexa Fluor® 555 Anti-EEF2 antibody [EP880Y]

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Rabbit Recombinant Monoclonal EEF2/Elongation factor 2 antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

EF2, EEF2, Elongation factor 2, EF-2

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-EEF2/Elongation factor 2 antibody [EP880Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-EEF2/Elongation factor 2 antibody [EP880Y]

  • 578 PE

    PE Anti-EEF2 antibody [EP880Y]

  • 660 APC

    APC Anti-EEF2 antibody [EP880Y]

  • HRP

    HRP Anti-EEF2 antibody [EP880Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-EEF2 antibody [EP880Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-EEF2/Elongation factor 2 antibody [EP880Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-EEF2/Elongation factor 2 antibody [EP880Y]

  • Unconjugated

    Anti-EEF2/Elongation factor 2 antibody [EP880Y]

  • Carrier free

    Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP880Y

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF2 or elongation factor 2 is an important protein in the process of translation during protein synthesis. It is also known by the names EF-2 and EF2. This protein facilitates the translocation step where it moves tRNA and mRNA from the A-site to the P-site on the ribosome. EEF2 has a molecular weight of approximately 95 kDa. The protein is ubiquitously expressed in various tissues and cells due to its fundamental role in protein synthesis making it essential for cellular activities.
Biological function summary

EEF2 functions as part of a larger ribosomal complex. It has an important role in ensuring the accuracy and efficiency of elongation during the translation process. This process is energy-dependent with EEF2 requiring GTP to catalyze the movement along the mRNA strand. The protein must undergo reversible phosphorylation which directly affects its activity. This post-translational modification of EEF2 is a significant modulation point influencing the rate of protein synthesis.

Pathways

EEF2 plays an integral role in the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway EEF2 activity is regulated to control protein translation based on cellular energy status and nutrient availability. This pathway is important for cell growth and proliferation. EEF2 interacts with other proteins such as factor 2 and eEF2kinase which phosphorylates the protein thereby inhibiting its activity. In the insulin signaling pathway similar regulatory mechanisms impact EEF2’s role in translation.

EEF2 connects to cancer and neurodegenerative diseases. In cancer aberrant regulation of EEF2 can lead to uncontrolled protein synthesis promoting cell proliferation and tumor growth. Its interaction with mTOR and other signaling proteins like ribosomal proteins plays a role in this dysregulation. In neurodegenerative conditions such as Alzheimer’s disease impaired EEF2 activity results in decreased synaptic protein synthesis affecting neuronal function. Understanding EEF2’s regulation and dysregulation provides insight into its involvement in these diseases offering potential for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed : 26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed : 26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed : 26593721).
See full target information EEF2
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Product promise

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