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Rabbit Recombinant Monoclonal Fos B antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Fos B antibody [EPR15905] (AB211854), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/100
Notes

This product gave a positive signal in TPA-treated HeLa cells fixed with 4% formaldehyde (10 min)

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Heterodimerizes with proteins of the JUN family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to gene promoters containing an AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing their transcriptional activity (PubMed:12618758, PubMed:28981703). As part of the AP-1 complex, facilitates enhancer selection together with cell-type-specific transcription factors by collaboratively binding to nucleosomal enhancers and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin (By similarity). Together with JUN, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Exhibits transactivation activity in vitro (By similarity). Involved in the display of nurturing behavior towards newborns (By similarity). May play a role in neurogenesis in the hippocampus and in learning and memory-related tasks by regulating the expression of various genes involved in neurogenesis, depression and epilepsy (By similarity). Implicated in behavioral responses related to morphine reward and spatial memory (By similarity). Isoform 11. Exhibits lower transactivation activity than isoform 1 in vitro (By similarity). The heterodimer with JUN does not display any transcriptional activity, and may thereby act as an transcriptional inhibitor (By similarity). May be involved in the regulation of neurogenesis in the hippocampus (By similarity). May play a role in synaptic modifications in nucleus accumbens medium spiny neurons and thereby play a role in adaptive and pathological reward-dependent learning, including maladaptive responses involved in drug addiction (By similarity). Seems to be more stably expressed with a half-life of ~9.5 hours in cell culture as compared to 1.5 hours half-life of isoform 1 (By similarity).

Alternative names

G0S3, FOSB, Protein FosB, G0/G1 switch regulatory protein 3, Transcription factor AP-1 subunit FosB

Recommended products

Rabbit Recombinant Monoclonal Fos B antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15905
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Fos B also known as FosB is a protein encoded by the FOSB gene and belongs to the Fos family of transcription factors. Weighing approximately 46 kDa this protein acts within the cell nucleus and is expressed in several tissues particularly in brain regions. Delta Fos B an isoform of Fos B has gained attention for its stable accumulation in certain neuronal populations. Researchers often study Fos B using techniques like ELISA and immunohistochemistry to investigate its presence and distribution.

Biological function summary

Functions of Fos B include regulation of gene expression through forming AP-1 transcription factor complexes with Jun family proteins. These complexes bind to DNA influencing the expression of genes involved in processes such as cell proliferation differentiation and survival. Fos B is structurally similar to other Fos family proteins such as c-Fos which emphasises its role in response to extracellular signals and stress stimuli. Both Fos B and its isoform Delta Fos B have distinct expression patterns suggesting unique functions.

Pathways

Fos B plays a role in the MAPK/ERK signaling pathway which is essential for transmitting extracellular signals to promote diverse cellular responses. Within this pathway interactions with proteins like Jun lead to modulation of gene expression critical for cellular responses to growth factors. Additionally connections to the CREB signaling pathway link Fos B to processes concerning neuronal plasticity and memory further highlighting its participation in important biological functions.

Associated diseases and disorders

Involvement of Fos B has been noted in neurological conditions such as addiction and depression. Delta Fos B in particular accumulates in response to chronic drug exposure and serves as a molecular switch in addiction-related neuroadaptations. Connections with proteins such as CREB and other transcriptional regulators have broadened the understanding of how Fos B and its isoforms impact neural circuits and contribute to the pathology of mood disorders.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Fos B antibody [EPR15905] (ab211854), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Fos B antibody [EPR15905] (ab211854)

    ab211854 staining Fos B in HeLa cells +/- TPA (200nM, 4 hours), then treated with LP (31 degrees C, 2 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

    The cells were then incubated overnight at +4°C with ab211854 at 1/100 dilution (pseudocolored in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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