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AB206900

Alexa Fluor® 555 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

5

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal H2A.X phospho S139 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p, γh2ax

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB206900)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB206900)

ab206900 staining Histone H2A.X (phospho S139) in Jurkat cells. The cells were incubated with 25μM Etoposide for 5 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab206900 at 1/100 dilution (shown in pseudo colour red) and ab195887 Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 488) at 2μg/ml (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

  • Carrier free

    Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free

  • Unconjugated

    Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • HRP

    HRP Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP854(2)Y

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Phospho-gamma H2A.X (S139) ELISA Kit

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
See full target information H2AX pS139
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2322068121 PubMed39042692

2024

Spatial molecular profiling of mixed invasive ductal and lobular breast cancers reveals heterogeneity in intrinsic molecular subtypes, oncogenic signatures, and mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Osama Shiraz Shah,Azadeh Nasrazadani,Julia Foldi,Jennifer M Atkinson,Celina G Kleer,Priscilla F McAuliffe,Tyler J Johnston,Wayne Stallaert,Edaise M da Silva,Pier Selenica,Higinio Dopeso,Fresia Pareja,Diana Mandelker,Britta Weigelt,Jorge S Reis-Filho,Rohit Bhargava,Peter C Lucas,Adrian V Lee,Steffi Oesterreich

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 64:1949-1955 PubMed37827841

2023

Immuno-PET and Targeted α-Therapy Using Anti-Glypican-1 Antibody Labeled with Zr or At: A Theranostic Approach for Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tadashi Watabe,Kazuya Kabayama,Sadahiro Naka,Ryuku Yamamoto,Kazuko Kaneda,Satoshi Serada,Kazuhiro Ooe,Atsushi Toyoshima,Yang Wang,Hiromitsu Haba,Kenta Kurimoto,Takanori Kobayashi,Eku Shimosegawa,Noriyuki Tomiyama,Koichi Fukase,Tetsuji Naka
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com