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AB216915

Alexa Fluor® 555 Anti-Gemin 3 antibody [EPR11283]

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Rabbit Recombinant Monoclonal Gemin 3 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

DP103, GEMIN3, DDX20, Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein 20, DEAD box protein DP 103, Gemin-3

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Gemin 3 antibody [EPR11283] (AB216915)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Gemin 3 antibody [EPR11283] (AB216915)

ab216915 staining Gemin 3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab216915 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11283

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gemin 3 also known as gemin3 is a protein that acts as an RNA helicase and plays an essential mechanical role in RNA processing and metabolism. It is part of the family of DEAD-box proteins and is highly conserved across species. The molecular mass of Gemin 3 is about 95 kDa. Expression of Gemin 3 occurs in various tissues with significant presence in the brain spinal cord and other neuronal tissues indicating its important role in neurological function.
Biological function summary

Gemin 3 forms part of the large multiprotein complex known as the survival of motor neuron (SMN) complex. This complex participates in the assembly of small nuclear ribonucleoproteins (snRNPs) which are fundamental components of the spliceosome. Gemin 3 contributes to spliceosome biogenesis and the management of RNA processing activities which are essential for normal cellular functions and gene expression regulation.

Pathways

Gemin 3 is critically involved in the SMN-mediated snRNP assembly pathway and also plays a role in the regulation of mRNA splicing. In these pathways Gemin 3 closely interacts with other proteins in the SMN complex such as Gemin 2 and Gemin 4 which are important for the stability and function of the entire snRNP assembly process. These interactions are necessary for efficient pre-mRNA splicing ensuring proper genetic information flow and expression.

Gemin 3 is directly linked to spinal muscular atrophy (SMA) a neurodegenerative disorder caused by mutations in the SMN1 gene affecting the SMN complex. When the SMN complex malfunctions due to inadequate cooperation between SMN and Gemin 3 it leads to defective snRNP assembly causing loss of motor neurons and muscle wasting characteristic of SMA. Additionally altered Gemin 3 function can be associated with certain other neurodegenerative conditions where RNA processing is impaired highlighting the protein's importance in maintaining neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
See full target information DDX20
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Product promise

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