Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-Fr and reacts with Rat, Mouse samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
IHC-Fr | |
---|---|
Human | Predicted |
Mouse | Tested |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/100 | Notes This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min). |
Species Mouse | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
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GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Glial fibrillary acidic protein, GFAP
Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-Fr and reacts with Rat, Mouse samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
GFAP Immunohistochemistry (Frozen sections) staining using rabbit Anti-GFAP antibody
IHC image of GFAP staining in a section of frozen normal rat dentate gyrus.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201735 at 1/100 (pseudocolored in green) and counterstained using Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
GFAP Immunohistochemistry (Frozen sections) staining of Mouse cerebellum using rabbit Anti-GFAP antibody
Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti-P2Y12 (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] ab309607, 1/50 dilution, 10 µg/mL) (green), anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-NeuN (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, 1/100 dilution, 5 µg/mL) (white) on Frozen sections of mouse cerebellum. Nuclear DNA was labelled with DAPI (blue).
Image B: Frozen section of mouse cerebellum stained with anti- P2Y12 (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] ab309607, 1/50 dilution, 10 µg/mL).
Image C: Frozen section of mouse cerebellum stained with anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL).
Image D: Frozen section of mouse cerebellum stained with anti-NeuN (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, 1/100 dilution, 5 µg/mL).
Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. The section was initially fixed using 10% formaldehyde in 1XPBS for 10 minutes. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.
Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cerebellum using rabbit Anti-GFAP antibody
Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti-NeuN (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195, 1/100 dilution, 5 µg/mL) (green), anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] - Synaptic Marker ab196166, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).
Image B: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-NeuN (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195, 1/100 dilution, 5 µg/mL).
Image C: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL).
Image D: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] - Synaptic Marker ab196166, 1/100 dilution, 5 µg/mL).
Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.
Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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