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Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-Fr and reacts with Rat, Mouse samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201735), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201735), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201735), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-Fr
Human
Predicted
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min).

Species
Mouse
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

2 products for Alternative Product

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Glial fibrillary acidic protein, GFAP

Recommended products

Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-Fr and reacts with Rat, Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1034Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201735), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201735)

    GFAP Immunohistochemistry (Frozen sections) staining using rabbit Anti-GFAP antibody

    IHC image of GFAP staining in a section of frozen normal rat dentate gyrus.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201735 at 1/100 (pseudocolored in green) and counterstained using Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201735), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201735)

    GFAP Immunohistochemistry (Frozen sections) staining of Mouse cerebellum using rabbit Anti-GFAP antibody

    Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti-P2Y12 (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] ab309607, 1/50 dilution, 10 µg/mL) (green), anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-NeuN (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, 1/100 dilution, 5 µg/mL) (white) on Frozen sections of mouse cerebellum. Nuclear DNA was labelled with DAPI (blue).

    Image B: Frozen section of mouse cerebellum stained with anti- P2Y12 (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] ab309607, 1/50 dilution, 10 µg/mL).

    Image C: Frozen section of mouse cerebellum stained with anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL).

    Image D: Frozen section of mouse cerebellum stained with anti-NeuN (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, 1/100 dilution, 5 µg/mL).

    Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. The section was initially fixed using 10% formaldehyde in 1XPBS for 10 minutes. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.

    Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201735), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201735)

    GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cerebellum using rabbit Anti-GFAP antibody

    Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti-NeuN (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195, 1/100 dilution, 5 µg/mL) (green), anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] - Synaptic Marker ab196166, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).

    Image B: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-NeuN (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195, 1/100 dilution, 5 µg/mL).

    Image C: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-GFAP (Alexa Fluor® 555) (ab201735, 1/100 dilution, 5 µg/mL).

    Image D: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] - Synaptic Marker ab196166, 1/100 dilution, 5 µg/mL).

    Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.

    Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com