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AB203432

Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

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(4 Publications)

Rabbit Recombinant Monoclonal HP1 alpha antibody - conjugated to Alexa Fluor® 555. Heterochromatin marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

HP1A, CBX5, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB203432)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB203432)

ab203432 staining HP1α in wild-type HAP1 cells (top panel) and HP1α in wild-type HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab203432 at 1/500 dilution (shown in pseudo colour green) and ab7291 at 1μg/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 647) (ab150119) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

This product also gave a positive signal under the same testing conditions in HAP1 cells fixed with 4% formaldehyde (10 min).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB203432)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (AB203432)

ab203432 staining HP1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203432 at a 1/50 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • 578 PE

    PE Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • HRP

    HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • Unconjugated

    Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

  • Carrier free

    Anti-HP1 alpha antibody [EPR5777] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5777

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HP1 alpha also known as HP1a or HP1 protein is a well-studied member of the heterochromatin protein 1 family. It plays a mechanical role in chromatin organization and gene regulation by binding to histone H3 when it's methylated at lysine 9 (H3K9me). This protein has a molecular mass of approximately 22-25 kDa. HP1 alpha is commonly expressed in the nuclei of eukaryotic cells where it localizes to heterochromatin regions and influences chromatin compaction.
Biological function summary

Heterochromatin protein 1 alpha acts as a modulator of gene silencing. It is part of a larger protein complex that contains various chromatin modifiers responsible for maintaining the silent state of certain genes. By mediating these interactions HP1 alpha helps regulate transcription replication timing and DNA repair processes. This regulation is essential for the structural maintenance of heterochromatin and plays a part in chromosomal stability.

Pathways

HP1 alpha is integral in the epigenetic regulation pathway along with other well-known proteins including SUV39H1 and SUV39H2 which are histone methyltransferases. HP1 alpha also links to the DNA damage response pathway where it recruits repair factors to maintain genomic integrity. Through these pathways HP1 alpha interacts closely with proteins like BRCA1 emphasizing its role in cellular homeostasis and response to DNA damage.

Researchers associate HP1 alpha with cancer and developmental disorders. Alterations in HP1 alpha expression or function can lead to disruptions in chromatin organization contributing to oncogenesis by affecting tumor suppressor genes. Furthermore HP1 alpha links to disorders like ICF syndrome through its relationship with the DNA methylation process where it interacts with proteins such as DNMT3B. Understanding these connections provides insights into therapeutic approaches targeting epigenetic modifications in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression (PubMed : 40440427). In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) (PubMed : 19783980). Also recognizes and binds histone H1.4 methylated at 'Lys-26' (H1.4K26me) (PubMed : 16127177). Excluded from chromatin when histone H1.4 is Simultaneously methylated at Lys-26 (H1.4K26me) and phosphorylated at Ser-27 (H1.4S27Ph) (PubMed : 16127177). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR) (PubMed : 19783980). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1 (PubMed : 19783980, PubMed : 20231385). Required for the formation of the inner centromere (PubMed : 20231385).
See full target information CBX5
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6420 PubMed40645983

2025

Forced expression of MSR repeat transcripts above a threshold limit breaks heterochromatin organisation.

Applications

Unspecified application

Species

Unspecified reactive species

Reagan W Ching,Kalina M Świst-Rosowska,Galina Erikson,Birgit Koschorz,Bettina Engist,Thomas Jenuwein

Nature communications 12:6241 PubMed34716321

2021

Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Ji Cha,Özgün Uyan,Yan Kai,Tianxin Liu,Qian Zhu,Zuzana Tothova,Giovanni A Botten,Jian Xu,Guo-Cheng Yuan,Job Dekker,Stuart H Orkin

eLife 10: PubMed34161229

2021

Multiple pathways of toxicity induced by dipeptide repeat aggregates and GC RNA in a cellular model.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric Frottin,Manuela Pérez-Berlanga,F Ulrich Hartl,Mark S Hipp

Nature 572:543-548 PubMed31391587

2019

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Eric Guo,John C Manteiga,Jonathan E Henninger,Benjamin R Sabari,Alessandra Dall'Agnese,Nancy M Hannett,Jan-Hendrik Spille,Lena K Afeyan,Alicia V Zamudio,Krishna Shrinivas,Brian J Abraham,Ann Boija,Tim-Michael Decker,Jenna K Rimel,Charli B Fant,Tong Ihn Lee,Ibrahim I Cisse,Phillip A Sharp,Dylan J Taatjes,Richard A Young
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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