JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313138

Alexa Fluor® 555 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal JAK1 phospho Y1034 + Y1035 antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

JAK1A, JAK1B, JAK1, Tyrosine-protein kinase JAK1, Janus kinase 1, JAK-1

  • HRP

    HRP Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

  • Unconjugated

    Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

  • Carrier free

    Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1899(2)

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

According to our ELISA result, this antibody preferentially recognizes JAK1 with phospho Y1035, but also recognizes JAK1 with phospho Y1034 if high amount peptides were coated.

style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JAK1 also known as Janus Kinase 1 plays an essential role in transmitting signals within cells. It is a tyrosine kinase enzyme with a molecular weight of approximately 130 kDa. JAK1 is expressed broadly in various tissues and is involved in cytokine receptor signaling. The kinase activity of JAK1 gets activated upon cytokine binding to its respective receptor which leads to phosphorylation events and downstream signaling cascades.
Biological function summary

This enzyme participates significantly in the immune response and hematopoiesis by being part of the JAK-STAT signaling pathway complex. When activated JAK1 phosphorylates specific tyrosine residues on the receptor creating docking sites for STAT proteins which then get phosphorylated by JAK1. This phosphorylation allows STAT proteins to dimerize and translocate to the nucleus affecting gene expression involved in growth survival and differentiation processes.

Pathways

JAK1 is importantly engaged in the JAK-STAT pathway and the Interferon signaling pathway. Within these pathways JAK1 collaborates closely with other proteins like JAK2 TYK2 and STAT proteins. It acts as a mediator of the cellular response to a variety of cytokines and growth factors playing a role in the regulation of immune responses and cell proliferation.

Dysregulation of JAK1 associates with autoimmune diseases like rheumatoid arthritis and certain cancers. JAK1 inhibitors have been developed as therapeutic agents for these conditions targeting its kinase activity to modulate aberrant signaling. The interaction between JAK1 and other kinases such as JAK2 and transcription factors like STATs highlights its importance in disease mechanisms providing a target for drugs to manage inflammatory and malignant disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed : 16239216, PubMed : 28111307, PubMed : 32750333, PubMed : 7615558, PubMed : 8232552). Kinase partner for the interleukin (IL)-2 receptor (PubMed : 11909529) as well as interleukin (IL)-10 receptor (PubMed : 12133952). Kinase partner for the type I interferon receptor IFNAR2 (PubMed : 16239216, PubMed : 28111307, PubMed : 32750333, PubMed : 7615558, PubMed : 8232552). In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins (PubMed : 7759950). Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors (PubMed : 16239216, PubMed : 32750333, PubMed : 8232552).
See full target information JAK1 phospho Y1034 + Y1035
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Cellular Activity - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

  • 5
  • 11
Assay Kits

AB279838

Phospho-JAK1 (Y1022) and Total JAK1 ELISA Kit

Sandwich ELISA - Phospho-JAK1 (Y1022) and Total JAK1 ELISA Kit (AB279838)

  • 0
  • 0
Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com