Skip to main content

Rabbit Recombinant Monoclonal Ki67 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Immunocytochemistry - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (AB215226), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (AB215226), expandable thumbnail
  • Immunocytochemistry - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (AB215226), expandable thumbnail

Publications

  • Micromachines 13:2022
    A Cell Culture Chip with Transparent, Micropillar-Decorated Bottom for Live Cell Imaging and Screening of Breast Cancer Cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Menekse Ermis,Ezgi Antmen,Ozgur Kuren,Utkan Demirci,Vasif Hasirci
    PubMed 35056257

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 - 20 min) and 100% methanol (5 min).

If fixing cells in 4% PFA, it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

    8 products for Alternative Version

      Target data

      Function

      Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

      Alternative names

      Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

      Recommended products

      1. Loading...
      2. Loading...
      3. Loading...
      4. Loading...

      Rabbit Recombinant Monoclonal Ki67 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

      Alternative names

      Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

      Key facts

      Isotype

      IgG

      Conjugation

      Alexa Fluor® 555

      Excitation/Emission

      Ex: 555nm, Em: 565nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR3610

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Immunocytochemistry - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226), expandable thumbnail

        Immunocytochemistry - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226)

        ab215226 staining Ki67 in wild-type HAP1 cells (top panel) and Ki67 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-Aquaporin 5 antibody [EPR3747] ab215225 at a 1/500 dilution (pseudocolored in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal [YOL1/34] to Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). This product also gave a positive signal under the same testing conditions in HAP1 cells fixed with 4% formaldehyde (10 min).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226), expandable thumbnail

        Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226)

        Overlay histogram showing HeLa cells stained with ab215226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215226, 1/500 dilution) for 30 min at 22°C.

        Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 555 (Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208569) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

        Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

      • Immunocytochemistry - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226), expandable thumbnail

        Immunocytochemistry - Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226)

        ab215226 staining Ki67 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215226 at 1/100 dilution (pseudocolored in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

        This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

      Downloads

      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com