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Rabbit Recombinant Monoclonal LC3B antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-P, ICC/IF and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Mouse
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed:30420355).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal LC3B antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18709

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

Associated diseases and disorders

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 μg/ml).

    Cytoplasmic staining on human cerebrum.

    The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 μg/ml).
    Cytoplasmic staining on human glioma.
    The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 μg/ml).

    Negative control: no staining on human stomach.


    The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling LC3B with ab307768 at 1/50 dilution (10 μg/ml) (Red).

    Confocal image showing increased cytoplasmic staining in HeLa cells treated with Chloroquine (50μM) for 24 hours.

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Green). The nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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