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AB210653

Alexa Fluor® 555 Anti-Mad2L2/REV7 antibody [EPR13657]

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(1 Publication)

Rabbit Recombinant Monoclonal Mad2L2/REV7 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MAD2B, REV7, MAD2L2, Mitotic spindle assembly checkpoint protein MAD2B, Mitotic arrest deficient 2-like protein 2, REV7 homolog, MAD2-like protein 2, hREV7

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Mad2L2/REV7 antibody [EPR13657] (AB210653)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Mad2L2/REV7 antibody [EPR13657] (AB210653)

ab210653 staining Mad2L2/REV7 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210653 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Mad2L2/REV7 antibody [EPR13657]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Mad2L2/REV7 antibody [EPR13657]

  • 578 PE

    PE Anti-Mad2L2 antibody [EPR13657]

  • 660 APC

    APC Anti-Mad2L2 antibody [EPR13657]

  • HRP

    HRP Anti-Mad2L2 antibody [EPR13657]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Mad2L2 antibody [EPR13657]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Mad2L2/REV7 antibody [EPR13657]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Mad2L2/REV7 antibody [EPR13657]

  • Unconjugated

    Anti-Mad2L2/REV7 antibody [EPR13657]

  • Carrier free

    Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13657

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB115622

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Mad2L2 also known as REV7 plays important roles in cellular processes. It has a molecular weight of about 26 kDa and is expressed in various tissues including the testis and placenta. Mad2L2 is an important component of the DNA damage response functioning mainly in the regulation of mitosis and repair of DNA double-strand breaks. It acts as a regulatory subunit and inhibitor of the translesion DNA synthesis (TLS) polymerase complex influencing processes linked to DNA fidelity.
Biological function summary

The protein is important for maintaining genome integrity by participating in the Fanconi anemia (FA) pathway ensuring proper DNA repair. Mad2L2 forms an important part of the homologous recombination repair system and plays a role in the recruitment of additional repair factors. It is often seen as a part of the shieldin complex which works to protect DNA ends during repair. This complex safeguarding allows it to prevent inappropriate activity during the DNA repair process.

Pathways

The involvement of Mad2L2 highlights its critical role in DNA repair mechanisms specifically homologous recombination and non-homologous end joining (NHEJ). It interacts with proteins such as 53BP1 and RIF1 within these pathways to mediate DNA repair. Such interaction is essential for choosing the correct repair pathway based on cellular needs. Mad2L2's function within the pathway guarantees the accurate resolution and repair of DNA lesions.

The protein's relevance underlines its connection to cancer and genetic disorders like Fanconi anemia. Mutations or irregularities in Mad2L2 can contribute to genomic instability leading to cancer development. In particular its interaction with the BRCA1 and BRCA2 proteins is significant; disruptions in these interactions link to increased cancer risk. This connection with tumor suppression pathways makes Mad2L2 an interesting target for therapeutic strategies in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein able to interact with different proteins and involved in different biological processes (PubMed : 11459825, PubMed : 11459826, PubMed : 17296730, PubMed : 17719540, PubMed : 19443654, PubMed : 29656893). Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis (PubMed : 20164194, PubMed : 23143872). Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions (PubMed : 20164194). Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs) (PubMed : 29656893). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection (PubMed : 29656893). Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres (PubMed : 29656893). May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1 (PubMed : 17296730). Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle (PubMed : 11459825, PubMed : 17719540). Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation (PubMed : 19443654).
See full target information MAD2L2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38791392

2024

Mesothelioma-Associated Fibroblasts Modulate the Response of Mesothelioma Patient-Derived Organoids to Chemotherapy via Interleukin-6.

Applications

Unspecified application

Species

Unspecified reactive species

Mario Cioce,Veronica Gatti,Fabiana Napolitano,Noemi Maria Giorgiano,Andrea Marra,Giuseppe Portella,Alfonso Fiorelli,Francesca Pentimalli,Vito Michele Fazio
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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