JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB312147

Alexa Fluor® 555 Anti-Mitofilin antibody [EPR8749]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Mitofilin antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

HMP, MIC60, MINOS2, PIG4, PIG52, IMMT, MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein, Mitochondrial inner membrane protein, Mitofilin, p87/89

  • 578 PE

    PE Anti-Mitofilin antibody [EPR8749]

  • 660 APC

    APC Anti-Mitofilin antibody [EPR8749]

  • HRP

    HRP Anti-Mitofilin antibody [EPR8749]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Mitofilin antibody [EPR8749]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Mitofilin antibody [EPR8749]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Mifilin antibody [EPR8749]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Mitofilin antibody [EPR8749]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Mitofilin antibody [EPR8749]

  • Unconjugated

    Anti-Mitofilin antibody [EPR8749]

  • Carrier free

    Anti-Mitofilin antibody [EPR8749] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8749

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitofilin also known as mitofilin or MIM is a protein with a mass of approximately 88 kDa. This protein is integral to the inner mitochondrial membrane. Mitofilin is found in various tissues with high expression in the heart and skeletal muscle indicating its significant role in energy-demanding tissues. It is the central component of the mitochondrial inner membrane organizing system (MINOS) which plays a fundamental role in maintaining the mitochondrial architecture.
Biological function summary

Within the mitochondrial environment mitofilin contributes to maintaining cristae morphology which is essential for optimal mitochondrial respiration and energy production. As part of the MINOS complex mitofilin interacts with other proteins such as Mic60 and Mic10 stabilizing the structure of crista junctions. This process ensures efficient electron transport chain function ultimately supporting ATP synthesis. Without effective shaping of the cristae cellular energy metabolism deteriorates.

Pathways

Research has highlighted the importance of mitofilin in apoptosis and bioenergetics pathways. It interacts with proteins like OPA1 a dynamin-related GTPase important for mitochondrial fusion and energy metabolism. Additionally mitofilin influences the release of cytochrome c an important step in the initiation of apoptosis. Proper functioning of these pathways is critical for cellular balance and survival showing mitofilin’s broad impact on cellular processes.

Mitofilin has links to mitochondrial myopathies and cardiomyopathies. These disorders stem from impaired mitochondrial function significantly affecting muscle tissues and heart performance. Disruption in mitofilin expression or function can lead to compromised energy production contributing to these conditions. Furthermore mitofilin has associations with proteins like Drp1 involved in mitochondrial dynamics and this relationship can influence the progression and severity of mitochondrial-related diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed : 22114354, PubMed : 25781180, PubMed : 32567732, PubMed : 33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed : 22114354, PubMed : 25781180, PubMed : 32567732, PubMed : 33130824).
See full target information IMMT
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Proteins & Peptides

AB289748

Recombinant Rat Mitofilin protein

SDS-PAGE - Recombinant Rat Mitofilin protein (AB289748)

  • 0
  • 0
Primary Antibodies

AB198610

Alexa Fluor® 647 Anti-alpha Actinin 4 antibody [EPR2533(2)] - Cytoskeleton Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Actinin 4 antibody [EPR2533(2)] - Cytoskeleton Marker (AB198610)

  • 0
  • 0
Primary Antibodies

AB18427

FITC Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype control

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com