AB218714

Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904]

Name: Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904] (ab218714) | Abcam
Brand: Abcam Limited
SKU: AB218714
Price: 620 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal Mucin 5AC antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 10 publications.

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904] (AB218714)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904] (AB218714)

ab218714 staining Mucin 5AC in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218714 at 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

HRP

HRP Anti-Mucin 5AC antibody [EPR16904]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Mucin 5AC antibody [EPR16904]
Unconjugated

Anti-Mucin 5AC antibody [EPR16904]
Carrier free

Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free
Carrier free

Anti-Mucin 5AC antibody [EPR16904] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16904

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Human, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.

Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).

See full target information MUC5AC

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1598436 PubMed40621452

2025

Tumor-suppressing multi-enterobacteria and PD-1/PD-L1 immune checkpoint inhibitor combination improves the outcome of hepatocellular carcinoma therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjuan Wu,Yaohua Fan,Mingsheng Zhang,Xiaoguang Wang,Xuesong He,Xinwei Guo,Yangchen Liu,Chenxi Cao,Zhaoqun Deng

Proceedings of the National Academy of Sciences of the United States of America 121:e2320303121 PubMed39008691

2024

Tropism for ciliated cells is the dominant driver of influenza viral burst size in the human airway.

Applications

Unspecified application

Species

Unspecified reactive species

Shanley N Roach,Frances K Shepherd,Clayton K Mickelson,Jessica K Fiege,Beth K Thielen,Lauren M Pross,Autumn E Sanders,Jason S Mitchell,Mason Robertson,Brian T Fife,Ryan A Langlois

ACS omega 8:11614-11622 PubMed37008083

2023

Magnetically Powered Chitosan Milliwheels for Rapid Translation, Barrier Function Rescue, and Delivery of Therapeutic Proteins to the Inflamed Gut Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Osmond,Elizabeth Korthals,Coy J Zimmermann,Eric J Roth,David W M Marr,Keith B Neeves

Mucosal immunology 15:952-963 PubMed35840680

2022

Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1.

Applications

Unspecified application

Species

Unspecified reactive species

Helen Stölting,Laury Baillon,Rebecca Frise,Katie Bonner,Richard J Hewitt,Philip L Molyneaux,Mindy L Gore,Wendy S Barclay,Sejal Saglani,Clare M Lloyd

Cells 11: PubMed35269407

2022

Neutrophil Extracellular Traps Do Not Induce Injury and Inflammation in Well-Differentiated RSV-Infected Airway Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Rosalie S N Linssen,Adithya Sridhar,Giulia Moreni,Nicole N van der Wel,Job B M van Woensel,Katja C Wolthers,Reinout A Bem

Nature communications 12:4354 PubMed34272374

2021

SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Rémy Robinot,Mathieu Hubert,Guilherme Dias de Melo,Françoise Lazarini,Timothée Bruel,Nikaïa Smith,Sylvain Levallois,Florence Larrous,Julien Fernandes,Stacy Gellenoncourt,Stéphane Rigaud,Olivier Gorgette,Catherine Thouvenot,Céline Trébeau,Adeline Mallet,Guillaume Duménil,Samy Gobaa,Raphaël Etournay,Pierre-Marie Lledo,Marc Lecuit,Hervé Bourhy,Darragh Duffy,Vincent Michel,Olivier Schwartz,Lisa A Chakrabarti

Frontiers in pharmacology 12:639716 PubMed33935729

2021

Development of an Advanced Multicellular Intestinal Model for Assessing Immunomodulatory Properties of Anti-Inflammatory Compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Marescotti,Giuseppe Lo Sasso,Diego Guerrera,Kasper Renggli,Pedro A Ruiz Castro,Romain Piault,Vincent Jaquet,Fabian Moine,Karsta Luettich,Stefan Frentzel,Manuel C Peitsch,Julia Hoeng

Journal of fungi (Basel, Switzerland) 7: PubMed33803702

2021

Dexamethasone Creates a Suppressive Microenvironment and Promotes Invasion in a Human 3D Epithelial/Immune Respiratory Model.

Applications

Unspecified application

Species

Unspecified reactive species

Maureen K Luvanda,Wilfried Posch,Asma Noureen,Eliott Lafon,Viktoria Zaderer,Cornelia Lass-Flörl,Doris Wilflingseder

Current research in toxicology 1:56-69 PubMed34345837

2020

Comparison of the basic morphology and function of 3D lung epithelial cultures derived from several donors.

Applications

Unspecified application

Species

Unspecified reactive species

David Bovard,Albert Giralt,Keyur Trivedi,Laurent Neau,Petros Kanellos,Anita Iskandar,Athanasios Kondylis,Karsta Luettich,Stefan Frentzel,Julia Hoeng,Manuel C Peitsch

SLAS technology 25:247-252 PubMed31971054

2020

In Vitro High-Content Imaging-Based Phenotypic Analysis of Bronchial 3D Organotypic Air-Liquid Interface Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Marescotti,David Bovard,Moran Morelli,Antonin Sandoz,Karsta Luettich,Stefan Frentzel,Manuel Peitsch,Julia Hoeng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904] (AB218714)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise