Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19]

8 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MX1 with ab307342 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on human tonsil.The section was incubated with ab307342 at 4ЎгC overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount?.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Daudi (human Burkitt's lymphoma lymphoblast) cells labelling MX1 with ab307342 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in Daudi cells treatment with interferon alpha 1 (20 U/ml ) for 24 h.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours (Red) / Untreated control (Green) cells labelling MX1 with ab307342 at 1/500 dilution (0.1ug) (Red) and Green (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor? 555) (Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208569) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Immunohistochemical analysis of paraffin-embedded Human breast carcino tissue labeling MX1 with ab307342 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on human breast carcinoma.The section was incubated with ab307342 at 4ЎгC overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount?.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) cells treated with 20 U/ml IFN alpha 1 for 24 hours (Red) / Untreated control (Green) cells labelling MX1 with ab307342 at 1/500 dilution (0.1ug) (Red) and Green (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208569) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A at 1/ was used as the secondary antibody.

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Daudi (human Burkitt's lymphoma lymphoblast) cells labelling MX1 with ab307342 at 1/50 (10.0 ug/ml) dilution, followed by antibody at 1/None dilution (Green). Confocal image showing cytoplasmic staining in Daudi cells treatment with interferon alpha 1 (20 U/ml ) for 24 h. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Positive staining on human breast carcinoma with ab307342 at 1/100 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The section was incubated with ab307342 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-MX1 antibody [EPR24485-19] (ab307342)

  Positive staining on human tonsil with ab307342 at 1/100 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The section was incubated with ab307342 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).