Rabbit Recombinant Monoclonal NeuroD1 antibody - conjugated to Alexa Fluor® 555.
IgG
Rabbit
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
BHLHA3, NEUROD, NEUROD, NEUROD1, BHLHA3, Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3
Rabbit Recombinant Monoclonal NeuroD1 antibody - conjugated to Alexa Fluor® 555.
IgG
Rabbit
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR4008
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This supplementary information is collated from multiple sources and compiled automatically.
NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.
NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.
NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.
Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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