Rabbit Recombinant Monoclonal PDK1 antibody - conjugated to Alexa Fluor® 555.
IgG
Rabbit
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
PDHK1, PDK1, PDHK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1
Rabbit Recombinant Monoclonal PDK1 antibody - conjugated to Alexa Fluor® 555.
IgG
Rabbit
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR19571
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This supplementary information is collated from multiple sources and compiled automatically.
The PDK1 protein also known as 3-phosphoinositide-dependent protein kinase-1 is a kinase enzyme coded by the PDPK1 gene. PDK1 has a molecular weight of approximately 63 kDa. It is expressed across a variety of tissues including high levels in the heart pancreas liver skeletal muscle and in certain brain regions. This kinase plays a pivotal role in cellular signaling pathways due to its ability to phosphorylate other proteins.
PDK1 acts as a master regulator of the AGC family of kinases which includes proteins like PKB/Akt S6K and SGK. By phosphorylating these proteins PDK1 controls cell growth glycogen synthesis and apoptosis. It does not function within a complex but operates as an important activator in phosphorylation events influencing several downstream cellular responses. By modulating kinase activity PDK1 significantly impacts cellular metabolism and survival pathways.
PDK1 serves as a critical component in the PI3K/Akt signaling pathway and the mTOR pathway. In the PI3K/Akt pathway PDK1 phosphorylates and activates Akt leading to varied outcomes such as glucose uptake cell proliferation and resistance to apoptosis under stressful conditions. The relationship between PDK1 and other proteins like mTOR highlights its importance in regulating cell growth and survival through nutrient-sensing mechanisms.
PDK1 is heavily implicated in cancer development and diabetes. Aberrant activation of the PI3K/Akt/mTOR pathway often driven by mutations influencing PDK1 activity is a common feature in many cancers. PDK1's role in modulating insulin signaling also connects it to type 2 diabetes where insulin resistance is a characteristic issue. Through these pathways PDK1 interacts with oncogenes and proteins like PTEN further influencing disease progression and treatment outcomes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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