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AB311892

Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28]

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Rabbit Recombinant Monoclonal Peripherin antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

NEF4, PRPH1, PRPH, Peripherin, Neurofilament 4

4 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on rat olfactory bulb.
Panel B : anti-SCN9A stained on rat olfactory bulb.
Panel C : anti-Peripherin stained in neurons of rat olfactory bulb.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on mouse sciatic nerve.
Panel B : anti-SCN9A stained on mouse sciatic nerve.
Panel C : anti-Peripherin stained on mouse sciatic nerve.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on mouse olfactory bulb.
Panel B : anti-SCN9A stained on mouse olfactory bulb.
Panel C : anti-Peripherin stained in neurons of mouse olfactory bulb.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (AB311892)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on rat sciatic nerve.
Panel B : anti-SCN9A stained on rat sciatic nerve.
Panel C : anti-Peripherin stained on rat sciatic nerve.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • 578 PE

    PE Anti-Peripherin antibody [EPR23445-28]

  • HRP

    HRP Anti-Peripherin antibody [EPR23445-28]

  • Unconjugated

    Anti-Peripherin antibody [EPR23445-28]

  • 660 APC

    APC Anti-Peripherin antibody [EPR23445-28]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Peripherin antibody [EPR23445-28]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Peripherin antibody [EPR23445-28]

  • Carrier free

    Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Peripherin antibody [EPR23445-28]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Peripherin antibody [EPR23445-28]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23445-28

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peripherin also known as 8G2 or 7C5 is a protein expressed strongly in peripheral and certain central nervous system neurons. The mass of the peripherin protein is approximately 57 kDa. This intermediate filament protein structurally supports neurons aiding in their proper formation maintenance and regeneration. Peripherin localizes to the axons of mature neurons and serves important functions in the neuronal cytoskeleton and its stability.
Biological function summary

Peripherin impacts the nervous system's structure and dynamics. As part of the cytoskeleton it contributes to neurofilament networks which are essential for neuronal shape and mechanical integrity. Peripherin plays a role in neuronal differentiation and apoptosis. It operates independently but occasionally interacts with other cytoskeletal proteins like neurofilament light protein to perform its duties in neuronal cells.

Pathways

Peripherin is involved in processes associated with neuronal development and injury response. It features in neurogenesis pathways where it influences the growth and repair of axons. Peripherin interacts with proteins such as vimentin which helps manage cell dynamics and stress responses. The axonal transport pathway also utilizes peripherin to support axonal transport under stressful conditions ensuring cellular integrity and function.

Peripherin connects strongly to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its overexpression or aggregation can create abnormal filamentous structures that contribute to motor neuron degeneration. In ALS peripherin may interact with TAR DNA-binding protein 43 (TDP-43) and superoxide dismutase 1 (SOD1) proteins known to be significant in the disease pathogenesis. Understanding peripherin’s role in these diseases aids in unraveling mechanisms underlying neuronal degeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Class-III neuronal intermediate filament protein (By similarity). May form an independent structural network without the involvement of other neurofilaments or may cooperate with the neuronal intermediate filament proteins NEFL, NEFH, NEFM and INA to form a filamentous network (PubMed : 15322088, PubMed : 15446584). Assembly of the neuronal intermediate filaments may be regulated by RAB7A (By similarity). Plays a role in the development of unmyelinated sensory neurons (By similarity). May be involved in axon elongation and axon regeneration after injury (By similarity). Inhibits neurite extension in type II spiral ganglion neurons in the cochlea (By similarity).
See full target information PRPH
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Product promise

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