Skip to main content

Rabbit Recombinant Monoclonal PFKM antibody - conjugated to Alexa Fluor® 555.

Be the first to review this product! Submit a review

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Target data

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Targets

PFKL

Alternative names

Rabbit Recombinant Monoclonal PFKM antibody - conjugated to Alexa Fluor® 555.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR11904

Purity

Tissue culture supernatant

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PFKM (phosphofructokinase muscle) and PFKL (phosphofructokinase liver) are key enzymes in glycolysis. PFKM is also known as phosphofructokinase-1 muscle type and has a mass of approximately 85 kDa while PFKL also has a similar mass. These enzymes are expressed mainly in their respective tissues—PFKM in muscle and PFKL in the liver—where they catalyze the conversion of fructose-6-phosphate to fructose-16-bisphosphate. This reaction is important for committing glucose to the glycolytic pathway controlling the rate of glycolysis.

Biological function summary

PFKM and PFKL play significant roles in cellular metabolism. They form homotetramers or heterotetramers in the presence of PFKP (phosphofructokinase platelet) another isozyme. The tetrameric enzyme complexes function as a significant control point for the glycolytic flux responding to allosteric activators like AMP and inhibitors such as ATP and citrate therefore regulating ATP production based on cellular energy demands.

Pathways

PFKM and PFKL are integral to glycolysis one of the primary metabolic pathways for energy production. They are involved in the regulation of energy homeostasis by interacting with other glycolytic enzymes such as hexokinase and pyruvate kinase. Their action ensures efficient conversion of glucose to pyruvate linking to the citric acid cycle and oxidative phosphorylation within aerobic respiration.

Associated diseases and disorders

PFKM and PFKL have connections to glycogen storage disease type VII (Tarui's disease) and cancer metabolism. In Tarui's disease mutations in the PFKM gene impair glycolysis in muscle tissues leading to exercise-induced muscle weakness. Furthermore cancer cells often exhibit altered PFKL expression attributed to their increased glycolytic rate for rapid proliferation. The interplay between these enzymes and regulation by p53 which can modulate PFKL activity influences glycolytic pathways in cancerous tissues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com