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AB203457

Alexa Fluor® 555 Anti-PMS2 antibody [EPR3947]

2

(2 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal PMS2 antibody - conjugated to Alexa Fluor® 555. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

PMSL2, PMS2, Mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 protein homolog 2

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-PMS2 antibody [EPR3947] (AB203457)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-PMS2 antibody [EPR3947] (AB203457)

Overlay histogram showing HeLa cells stained with ab203457 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab203457, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 555 (ab208569) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3947

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PMS2 also known as Postmeiotic Segregation Increased 2 or PMS2 MutL homolog is a protein that participates in DNA mismatch repair. It weighs approximately 96 kDa and often identifies as a member of the MutL protein family. PMS2 is ubiquitously expressed in the body with higher abundances in tissues that undergo rapid proliferation or possess a high mitotic index.
Biological function summary

PMS2 operates as part of the DNA mismatch repair (MMR) complex. It collaborates with other MutL homologs including MLH1 to form a heterodimer which is essential for repairing DNA replication errors. It safeguards genomic integrity and prevents mutations from accumulating in dividing cells serving important functions in cellular viability and genetic stability.

Pathways

PMS2 is involved in the DNA damage response and cell cycle regulation. The protein plays a vital role in the mismatch repair (MMR) pathway. PMS2 partners primarily with MLH1 within this pathway and both proteins work in conjunction to recognize and initiate repair on erroneous DNA sequences that emerge during replication preventing illegitimate recombination and chromosomal rearrangements.

PMS2 mutations occur frequently in Lynch syndrome an inherited cancer predisposition disorder and Turcot syndrome a condition associated with colorectal cancer and brain tumors. MLH1 frequently associates with PMS2 in these disorders as defects in either protein can impair mismatch repair leading to an increased risk of cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the post-replicative DNA mismatch repair system (MMR) (PubMed : 30653781, PubMed : 35189042). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair (PubMed : 35189042).
See full target information PMS2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2403917121 PubMed38980903

2024

Intratumoral NKT cell accumulation promotes antitumor immunity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayun Li,Philip Moresco,Douglas T Fearon

Cancer research communications 4:1369-1379 PubMed38709075

2024

Pan-Cancer Interrogation of B7-H3 (CD276) as an Actionable Therapeutic Target Across Human Malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Carly D Miller,John R Lozada,Nicholas A Zorko,Andrew Elliott,Allison Makovec,Milan Radovich,Elisabeth I Heath,Neeraj Agarwal,Rana R Mckay,Rohan Garje,Bruno R Bastos,Dave S B Hoon,Jacob J Orme,Oliver Sartor,Ari VanderWalde,Chadi Nabhan,George Sledge,Eugene Shenderov,Scott M Dehm,Emil Lou,Jeffrey S Miller,Justin H Hwang,Emmanuel S Antonarakis

Clinical cancer research : an official journal of the American Association for Cancer Research 29:2702-2713 PubMed37126020

2023

Metastatic Prostate Cancers with BRCA2 versus ATM Mutations Exhibit Divergent Molecular Features and Clinical Outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Justin Hwang,Xiaolei Shi,Andrew Elliott,Taylor E Arnoff,Julie McGrath,Joanne Xiu,Phillip Walker,Hannah E Bergom,Abderrahman Day,Shihab Ahmed,Sydney Tape,Allison Makovec,Atef Ali,Rami M Shaker,Eamon Toye,Rachel Passow,John R Lozada,Jinhua Wang,Emil Lou,Kent W Mouw,Benedito A Carneiro,Elisabeth I Heath,Rana R McKay,W Michael Korn,Chadi Nabhan,Charles J Ryan,Emmanuel S Antonarakis
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com