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Anti-Rabbit IgG, monoclonal [EPR25A] - Isotype Control- Alexa Fluor® 555 conjugated (ab208569) is a rabbit recombinant monoclonal antibody used in Flow Cytometry, ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208569), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208569), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208569), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208569), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208569), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

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12 products for Alternative Version

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Anti-Rabbit IgG, monoclonal [EPR25A] - Isotype Control- Alexa Fluor® 555 conjugated (ab208569) is a rabbit recombinant monoclonal antibody used in Flow Cytometry, ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Form
Liquid
Clonality
Monoclonal
Clone number
EPR25A
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

What is this antibody validated in?


Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF).


Other related products


We have a range of other formats of antibody clone [EPR25A] also available for your convenience:
Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Alexa Fluor® 488 - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091, Alexa Fluor® 647 - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093, Carrier free - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) ab199376, HRP - HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199507, Alexa Fluor® 405 - Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208150, Alexa Fluor® 594 - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208568, Alexa Fluor® 555 - ab208569, PE - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478, Alexa Fluor® 568 - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209613, Carrier free - Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free ab210849, PerCP - PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab222107, FITC - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab223339, APC - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814, Biotin - Biotin Isotype Control [EPR25A] ab320073, Alexa Fluor® 750 - Alexa Fluor® 750 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab322086



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208569 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/1000 dilution (showing no signal) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CXCL16 with Alexa Fluor® 555 Anti-CXCL16 antibody [EPR29713-506] ab324079 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) / Left isotype control.

    Cells were co-stained with CD11b conjugated BV421.

    Gated on viable cell.

  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of Isotype (Left) / 293T cells transfected with a human EMR1/ADGRE1 expression vector containing a myc tag (Middle) / 293T cells transfected with an empty vector containing a myc tag (Right) cells labelling EMR1/ADGRE1 with Alexa Fluor® 555 Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] ab316877 at 1/50 dilution (1 ug)/Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cell.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric (intracellular) analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, left) / Ramos (human Burkitt's lymphoma B lymphocyte, right) cell lines labelling CD20 with Alexa Fluor® 555 Anti-CD20 antibody [EP459Y] ab312151 at 1/5000 dilution (red) compared with Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) (black) and an unlabelled control (cell without incubation with primary antibody and secondary antibody) (blue).

    10% Goat serum was used as a blocking agent.

    Negative control: HEK-293.

  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling TREM1 with Alexa Fluor® 555 Anti-TREM1 antibody [EPR26207-34] ab316942 at 1/50 dilution (1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Human PBMC are co-stained with CD11b conjugated BV421.

    Gated on viable cell.

  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling TREM1 with Alexa Fluor® 555 Anti-TREM1 antibody [EPR26207-34] ab316942 at 1/50 dilution (1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Human PBMC are co-stained with CD3 conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4(human lymphoblastic leukemia T lymphoblast) cells labelling Notch1 with Alexa Fluor® 555 Anti-Notch1 antibody [EP1238Y] ab315420 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell) cells labelling Notch1 with Alexa Fluor® 555 Anti-Notch1 antibody [EP1238Y] ab315420 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling CD24 with Alexa Fluor® 555 Anti-CD24 antibody [EPR19925] ab315293 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Negative control: MDA-MB-231.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Overlay histogram showing HeLa cells stained with ab202520 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab202520, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 555 (ab208569) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized B16F0 (mouse melanoma epithelial cell-like) cells labeling SOX10 with Alexa Fluor® 555 Anti-SOX10 antibody [SP267] ab307763 at 1/50 dilution (1.0µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CXCL16 with Alexa Fluor® 555 Anti-CXCL16 antibody [EPR29713-506] ab324079 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) / Left isotype control.

    Cells were co-stained with CD19 conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) (Left panel) / A375 (human malignant melanoma epithelial cell) (Right panel) cells labeling SOX10 with Alexa Fluor® 555 Anti-SOX10 antibody [SP267] ab307763 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Negative control: HeLa.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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