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AB311867

Alexa Fluor® 555 Anti-Rad21 antibody [EPR22506-15]

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Rabbit Recombinant Monoclonal RAD21 antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

HR21, KIAA0078, NXP1, SCC1, RAD21, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1

  • 660 APC

    APC Anti-Rad21 antibody [EPR22506-15]

  • HRP

    HRP Anti-Rad21 antibody [EPR22506-15]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Rad21 antibody [EPR22506-15]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Rad21 antibody [EPR22506-15]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Rad21 antibody [EPR22506-15]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Rad21 antibody [EPR22506-15]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

  • Unconjugated

    Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

  • Carrier free

    Anti-Rad21 antibody [EPR22506-15] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22506-15

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rad21 also known as double-strand-break repair protein rad21 homolog serves as a component of the cohesin complex. This protein is approximately 123 kDa in size. Rad21 is expressed in a wide range of tissues and cell types particularly in those with high proliferative activity. The protein plays an essential mechanical role by facilitating the cohesion of sister chromatids during cell division ensuring successful chromosome segregation.
Biological function summary

Rad21 is essential in processes beyond chromatid cohesion. It forms part of the cohesin complex which also includes proteins like SMC1 SMC3 and SCC3/SA1 or SA2. This complex is central to maintaining genome stability by participating in DNA repair and regulating gene expression. Through these roles Rad21 contributes to cellular processes such as cell cycle progression and embryonic development.

Pathways

Rad21 integrates into the DNA damage response and cell cycle regulation pathways. The cohesin complex with Rad21 as an important factor acts alongside other proteins such as ATM and ATR in the DNA damage response ensuring the repair of double-strand breaks. Within the cell cycle Rad21 helps coordinate the timing and accuracy of cell division working with cyclin-dependent kinases.

Rad21 has been linked to cancer and Cornelia de Lange syndrome. Mutations or dysregulation of Rad21 can result in tumorigenesis due to errors in chromosome segregation and compromised DNA repair. In Cornelia de Lange syndrome cohesin-related proteins like NIPBL and SMC1A also play a role indicating a disruption in the cohesin pathway's function. Understanding Rad21's involvement helps in developing targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Double-strand-break repair protein rad21 homolog. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed : 11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed : 11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed : 25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).. 64-kDa C-terminal product. May promote apoptosis.
See full target information RAD21
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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