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AB312159

Alexa Fluor® 555 Anti-RHEB antibody [EPR2971]

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Rabbit Recombinant Monoclonal RHEB antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

RHEB2, RHEB, GTP-binding protein Rheb, Ras homolog enriched in brain

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2971

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RHEB also known as Ras homolog enriched in brain is a small GTPase with a molecular mass of approximately 21 kDa. It is a member of the Ras superfamily and plays a role in the regulation of cell growth and proliferation. RHEB is widely expressed with concentrations found in the brain skeletal muscle and various other tissues. It functions as a molecular switch cycling between active GTP-bound and inactive GDP-bound states influencing signaling pathways linked to growth and metabolism.
Biological function summary

RHEB interacts with several components within the mTORC1 complex a major regulator of cell growth and autophagy. This protein positively regulates the mTORC1 pathway by activating mTOR leading to protein synthesis and cell growth when nutrients such as amino acids and energy are available. RHEB's regulation of mTORC1 is influenced by upstream modulation through the TSC1/2 complex acting as a sensor for cellular energy levels and growth signals. The role of RHEB in these regulatory mechanisms highlights its fundamental importance in cellular metabolic processes.

Pathways

RHEB plays a significant role in the mechanistic target of rapamycin (mTOR) signaling pathway which is important for regulating protein synthesis and cellular growth. RHEB acts downstream of the TSC1/2 complex with TSC2 acting as a GTPase-activating protein for RHEB. This interaction points RHEB as an essential regulator of mTORC1 activation. In connection with other proteins RHEB functions alongside mTOR and TSC1/2 to mediate responses to cellular nutrient status thereby adjusting growth and proliferation accordingly.

Mutations or dysregulation of RHEB have been linked to tuberous sclerosis complex (TSC) and cancer. In TSC where aberrant signaling occurs through the mTOR pathway RHEB's activity influences the mTORC1 pathway in cells lacking functional TSC1 or TSC2 leading to benign tumors. Additionally RHEB overexpression has been implicated in certain cancers due to its capacity to enhance cell proliferation via mTORC1. The interaction of RHEB with mTOR highlights its role in disease states where cell growth control is altered suggesting the potential for targeted therapies in treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 12172553, PubMed : 12271141, PubMed : 12842888, PubMed : 12869586, PubMed : 12906785, PubMed : 15340059, PubMed : 15854902, PubMed : 16098514, PubMed : 20381137, PubMed : 22819219, PubMed : 24529379, PubMed : 29416044, PubMed : 32470140, PubMed : 33157014). In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex : acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) (PubMed : 29236692, PubMed : 33157014). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes (PubMed : 24529379). In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1 (PubMed : 24529379, PubMed : 33157014). Has low intrinsic GTPase activity (PubMed : 15340059).
See full target information RHEB
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