JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB207253

Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal RUNX1 / AML1 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

AML1, CBFA2, RUNX1, Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, CBF-alpha-2, PEA2-alpha B, PEBP2-alpha B

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (AB207253)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (AB207253)

ab207253 staining RUNX1 / AML1+RUNX3+RUNX2 in Neuro2a cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207253 at a 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099]

  • 578 PE

    PE Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • 660 APC

    APC Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • HRP

    HRP Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • Unconjugated

    Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

  • Carrier free

    Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3099

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in Neuro2a cells fixed with 4% formaldehyde (10 min)</p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RUNX1 also known as AML1 is a transcription factor that plays an important role in gene regulation. RUNX3 and RUNX2 are related proteins that belong to the same RUNX family which are defined by the runt homology domain. RUNX1 typically has a mass of approximately 50 kilodaltons (kDa). These proteins express in various tissues including hematopoietic cells for RUNX1 skeletal tissues for RUNX2 and immune cells for RUNX3. They bind to DNA influencing the transcription of target genes which are critical for development processes.
Biological function summary

RUNX proteins mediate cellular differentiation and proliferation. They often participate in complex with CBFβ which stabilizes their interaction with DNA and enhances transcriptional activity. RUNX1 in particular regulates hematopoiesis the process of blood cell formation. RUNX2 is fundamentally involved in osteogenesis facilitating bone growth and remodeling. RUNX3 contributes to immune responses by affecting T-cell differentiation and function. Their dynamic interactions dictate the expression levels of genes involved in these vital processes.

Pathways

RUNX proteins integrate into significant signaling cascades. In hematopoietic regulation RUNX1 interacts importantly within the Notch and TGF-β pathways. It cooperates with other proteins such as GATA1 and TAL1 influencing lineage commitment and differentiation of blood cells. RUNX2 critical in the ossification pathway often partners with proteins including SMADs in response to bone morphogenetic protein (BMP) signaling guiding osteoblast differentiation. These pathways demonstrate the versatility and specificity of RUNX proteins in biological processes.

Aberrations in RUNX1 are strongly linked to acute myeloid leukemia (AML). Mutations or translocations affecting RUNX1 disrupt normal hematopoiesis leading to leukemogenic processes. RUNX3 involvement can be observed in cancers like gastric cancer where its silencing or inactivation associates with tumorigenesis. RUNX2 overexpression sometimes links with bone disorders such as cleidocranial dysplasia. Here RUNX2 may interact with other skeletal regulators like PTHLH influencing proper bone development and growth. Understanding these interactions provides insights into potential therapeutic targets for managing these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed : 17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed : 10207087, PubMed : 14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed : 17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity).. Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation.. Isoform AML-1L interferes with the transactivation activity of RUNX1.
See full target information RUNX1

Additional targets

RUNX2,RUNX3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific data 6:323 PubMed31848351

2019

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rumana Rashid,Giorgio Gaglia,Yu-An Chen,Jia-Ren Lin,Ziming Du,Zoltan Maliga,Denis Schapiro,Clarence Yapp,Jeremy Muhlich,Artem Sokolov,Peter Sorger,Sandro Santagata
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB133261

Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (AB133261)

  • 0
  • 1

Complementary Products

Assay Kits

AB282863

Human RUNX1 / AML1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human RUNX1 / AML1 ELISA Kit (AB282863)

  • 0
  • 0
Primary Antibodies

AB282004

Alexa Fluor® 488 Anti-Osteopontin antibody [EPR21139-316]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Osteopontin antibody [EPR21139-316] (AB282004)

  • 0
  • 0
Primary Antibodies

AB307523

Alexa Fluor® 647 Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Alexa Fluor® 647 Anti-CD11b antibody [EPR1344] (AB307523)

  • 0
  • 0
Primary Antibodies

AB215955

Alexa Fluor® 647 Anti-RUNX2 antibody [EPR14334]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RUNX2 antibody [EPR14334] (AB215955)

  • 0
  • 0
Reagents, Controls & Accessories

AB64220

Proteinase K Antigen Retrieval Solution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K Antigen Retrieval Solution (AB64220)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com