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AB217228

Alexa Fluor® 555 Anti-SCGB3A2 antibody [EPR11463]

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Rabbit Recombinant Monoclonal SCGB3A2 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

PNSP1, UGRP1, UNQ566/PRO1128, SCGB3A2, Secretoglobin family 3A member 2, Pneumo secretory protein 1, Uteroglobin-related protein 1, PnSP-1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-SCGB3A2 antibody [EPR11463] (AB217228)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-SCGB3A2 antibody [EPR11463] (AB217228)

ab217228 staining SCGB3A2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab217228 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11463

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCGB3A2 also recognized as uteroglobin-related protein 1 (UGRP1) is a small secreted protein with a molecular mass around 10 kDa. It is mainly expressed in the epithelial cells of the respiratory tract notably the lungs where it plays a role in modulating immune responses. In terms of structure SCGB3A2 shares similarities with other secretoglobin family members indicating conserved functional motifs. Its expression is tightly regulated and reflects its involvement in maintaining respiratory health.
Biological function summary

SCGB3A2 acts as an anti-inflammatory agent that modulates immune cell activity and cytokine signaling within the lungs. It does not form known protein complexes but interacts with other immune mediators to reduce inflammation. Furthermore SCGB3A2 has roles in cell proliferation and repair mechanisms especially in response to lung injury. Its interaction with various cytokines supports tissue regeneration and homeostasis making it important in maintaining lung function.

Pathways

SCGB3A2 interacts in immune regulatory pathways significant in lung tissue. Notably it participates in pathways related to the suppression of inflammatory cytokine production and has a working relationship with proteins such as IL-10 and TGF-beta that mitigate excessive immune reactions. These interactions exhibit roles in the regulation of immune responses and the promotion of tissue repair making SCGB3A2 part of important biological processes that protect lung integrity.

SCGB3A2 is linked to conditions such as asthma and chronic obstructive pulmonary disease (COPD) where inflammation plays an important role. Alterations in SCGB3A2 expression correlate with disease severity suggesting its potential as a biomarker. Its relationship with TGF-beta is of particular interest as dysregulation of this protein contributes to fibrotic changes in lung tissue. Studies continue to explore how modulating SCGB3A2 levels may influence disease progression and treatment outcomes in inflammatory lung disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Secreted cytokine-like protein (PubMed : 12847263). Binds to the scavenger receptor MARCO (PubMed : 12847263). Can also bind to pathogens including the Gram-positive bacterium L.monocytogenes, the Gram-negative bacterium P.aeruginosa, and yeast (PubMed : 12847263). Strongly inhibits phospholipase A2 (PLA2G1B) activity (PubMed : 24213919). Seems to have anti-inflammatory effects in respiratory epithelium (By similarity). Also has anti-fibrotic activity in lung (PubMed : 24213919). May play a role in fetal lung development and maturation (PubMed : 24213919). Promotes branching morphogenesis during early stages of lung development (PubMed : 24213919). In the pituitary, may inhibit production of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) (By similarity).
See full target information SCGB3A2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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