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AB324339

Alexa Fluor® 555 Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6]

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Rabbit Recombinant Monoclonal Pulmonary surfactant-associated protein A1 antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

COLEC4, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA1, Pulmonary surfactant-associated protein A1, PSP-A, PSPA, SP-A, SP-A1, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein, Collectin-4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB324339)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB324339)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SFTPA1 + SFTPA2 with ab324339 at 1/100 dilution.

Negative control : no staining on human kidney.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB324339)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB324339)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SFTPA1 + SFTPA2 with ab324339 at 1/100 dilution.

Negative control : no staining on human spleen.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB324339)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB324339)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling SFTPA1 + SFTPA2 with ab324339 at 1/100 dilution.

Positive staining on human lung.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Unconjugated

    Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6]

  • Carrier free

    Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29098-6

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen shares no sequence similarity with SFTPB, SFTPC or SFTPD. Based on the immunogen sequence, we do not expect this antibody to cross-react with SFTPB, SFTPC or SFTPD.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFTPA1 and SFTPA2 also known as surfactant protein A1 and A2 are essential components of the pulmonary surfactant system. SFTPA1 and SFTPA2 are glycoproteins with a molecular mass of approximately 26 kDa. These proteins are mainly expressed in the lung alveolar type II epithelial cells. They play a critical role in reducing surface tension in the alveoli preventing lung collapse during respiration. SFTPA1 and SFTPA2 bind to lipids within the surfactant helping to maintain its structure and function.
Biological function summary

SFTPA1 and SFTPA2 are involved in the immune defense mechanisms of the lungs. Both proteins are part of the collectin family and form higher-order oligomeric structures. As a component of the surfactant protein complex they enhance phagocytosis of pathogens by alveolar macrophages and neutralize pathogens directly. Additionally they modulate inflammatory responses in the lung therefore contributing to the protection of pulmonary tissues.

Pathways

SFTPA1 and SFTPA2 are integral to the innate immune response and lipid metabolism pathways in the lungs. They interact with toll-like receptors (TLRs) and other surfactant proteins like surfactant protein D (SFTPD) to activate signaling pathways that lead to inflammation control. SFTPA1 and SFTPA2 also influence surfactant homeostasis within the lung environment contributing to the maintenance of respiratory function.

SFTPA1 and SFTPA2 relate closely to respiratory distress syndrome (RDS) and pulmonary alveolar proteinosis (PAP). Mutations in SFTPA1 and SFTPA2 genes can impair surfactant function which often results in RDS particularly in premature infants. Moreover alterations in these proteins can also contribute to PAP where abnormal surfactant accumulation occurs. Their interplay with proteins like surfactant protein B (SFTPB) further links them to these pulmonary disorders as these proteins work together to ensure proper surfactant functionality.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages (By similarity).. (Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule (PubMed : 17158455, PubMed : 21203928). Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages (PubMed : 21123169).. (Microbial infection) Binds M.pneumoniae CARDS toxin, serves as one receptor for this pathogen (PubMed : 15845487, PubMed : 25139904). When SFTPA1 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen (PubMed : 25139904).
See full target information SFTPA1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com