AB318923

Alexa Fluor® 555 Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]

RabMAb
Recombinant
What is this?

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Rabbit Recombinant Monoclonal SMAD2 phospho T8 antibody - conjugated to Alexa Fluor® 555. Suitable for Target Binding Affinity, Antibody Labelling, ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

MADH2, MADR2, SMAD2, SMAD family member 2, SMAD 2, hSMAD2, JV18-1, Mad-related protein 2, Mothers against decapentaplegic homolog 2, hMAD-2, MAD homolog 2, Mothers against DPP homolog 2

578 PE

PE Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]
660 APC

APC Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]
Unconjugated

Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]
Carrier free

Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23682-64

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "TargetBindingAffinity-species-checked": "predicted", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "predicted", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 and Smad3 also known as Smad family member 2 and Smad family member 3 are intracellular proteins involved in signaling cascades. Smad2 has a molecular mass of approximately 58 kDa and Smad3 is around 52 kDa. These proteins are part of the Smad family which functions in the signal transduction for the Transforming Growth Factor-beta (TGF-β) superfamily. Smad2 and Smad3 are widely expressed in many tissues including the brain heart and lung suggesting their broad biological roles.

Biological function summary

Smad2 and Smad3 regulate transcriptional responses essential for cellular processes including proliferation differentiation and apoptosis. These proteins form a trimeric complex with Smad4 upon activation by TGF-β receptors. The activated complex translocates into the nucleus where it binds to DNA and influences gene expression. Such actions highlight their fundamental role in controlling cell behavior and fate.

Pathways

Smad2 and Smad3 play critical roles within the TGF-β signaling pathway. This pathway has significant effects on cell cycle control and immune regulation. Both Smad2 and Smad3 interact closely with the protein Smad4 to mediate various downstream effects integrating signals with other pathways such as MAPK pathway which involves different cellular responses.

Smad2 and Smad3 are closely associated with cancer and fibrosis. Aberrant TGF-β signaling often leads to tumor progression and metastasis partly due to the dysregulated actions of Smad2 and Smad3. Additionally these proteins through their pathway functions contribute to the pathological process of fibrosis in organs like the liver and lungs. It is important to note their interaction with the ERK protein which can also influence disease outcomes in these contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).

See full target information SMAD2 phospho T8

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64]

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise