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AB313143

AF555 Anti-SMAD3 (pS423/425) + SMAD5 & SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

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Rabbit Recombinant Monoclonal SMAD3 phospho S423 + S425 antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

  • 519 Alexa Fluor® 488

    AF488 Anti-SMAD3 (pS423/425) + SMAD5 & SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

  • 665 Alexa Fluor® 647

    AF647 Anti-SMAD3 (pS423/425) + SMAD5 & SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

  • 617 Alexa Fluor® 594

    AF594 Anti-SMAD3 (pS423/425) + SMAD5 & SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

  • 603 Alexa Fluor® 568

    AF568 Anti-SMAD3 (pS423/425) + SMAD5 & SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

  • 775 Alexa Fluor® 750

    A750 Anti-SMAD3 (pS423/425)+SMAD5 (pS463/465)+SMAD1 (pS463/465)+SMAD2 (pS465/467) antibody [EP823Y]

  • Unconjugated

    Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

  • Carrier free

    Anti-SMAD3 (pS423/425)+SMAD5&SMAD1 (pS463/465)+SMAD2 (pS465/467) antibody [EP823Y] - BSA&Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP823Y

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects Smad3 phosphorylated on Serine 423 and Serine 425. This Smad3 antibody may also detect Smad1, Smad2 and Smad5 phosphorylated at the equivalent sites.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.
Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.
See full target information SMAD3 phospho S423 + S425
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Product promise

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For full details, please see our Terms & Conditions

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