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AB312046

Alexa Fluor® 555 Anti-SMYD3 antibody [EPR11107(2)]

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Rabbit Recombinant Monoclonal SMYD3 antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

ZMYND1, ZNFN3A1, SMYD3, Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1

  • Unconjugated

    Anti-SMYD3 antibody [EPR11107(2)]

  • 578 PE

    PE Anti-SMYD3 antibody [EPR11107(2)]

  • 660 APC

    APC Anti-SMYD3 antibody [EPR11107(2)]

  • HRP

    HRP Anti-SMYD3 antibody [EPR11107(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-SMYD3 antibody [EPR11107(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-SMYD3 antibody [EPR11107(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-SMYD3 antibody [EPR11107(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-SMYD3 antibody [EPR11107(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SMYD3 antibody [EPR11107(2)]

  • Carrier free

    Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11107(2)

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMYD3 short for SET and MYND domain containing 3 is a member of the protein lysine methyltransferase family. It mainly functions as a histone methyltransferase adding methyl groups to lysine 4 on histone H3 (H3K4) and lysine 5 on histone H4 (H4K5) influencing chromatin structure and gene expression. SMYD3 has a molecular mass of approximately 56 kDa. It is widely expressed in multiple tissues but levels are higher in skeletal muscle and the heart. This broad expression indicates its potential role in various cellular functions.
Biological function summary

This methyltransferase regulates transcriptional activation by methylating histones. It acts as part of transcriptional coactivator complexes interacting with RNA polymerase II. SMYD3 modulates the expression of genes involved in cell proliferation and cancer progression. Additionally it interacts with proteins like Heat Shock Protein 90 (HSP90) and promotes the stabilization of substrate proteins. Its influence extends to non-histone proteins which suggests a wider impact on cellular signaling and structure.

Pathways

SMYD3 is involved in the MAPK (Mitogen-Activated Protein Kinase) signaling pathway an important regulator of cell differentiation proliferation and survival. It interacts with key proteins in this pathway such as ERK2 (Extracellular signal-regulated kinase 2) influencing cellular outcomes. SMYD3 also plays a role in the Wnt signaling pathway which is important for development and oncogenesis. This interaction with signaling pathways underlines its significant regulatory functions within the cell.

SMYD3 is strongly associated with cancer. Its overexpression contributes to tumorigenesis and metastasis particularly in colorectal and liver cancers. In cancer SMYD3 interacts with proteins like AKT1 promoting cancer cell survival and growth. Additionally SMYD3 has been linked to congenital heart diseases through its role in cardiac development. It interacts with cardiac transcription factors further connecting its function to heart malformations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed : 15235609, PubMed : 22419068). Also methylates 'Lys-5' of histone H4 (PubMed : 22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed : 15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed : 15235609).
See full target information SMYD3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB232626

Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free

primary-antibodies

smyd3-antibody-epr111072-bsa-and-azide-free-ab232626

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