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Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to Alexa Fluor® 555. Suitable for mIHC, ICC and reacts with Human samples.


Images

Immunocytochemistry - Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB274883), expandable thumbnail
  • Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB274883), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCICC
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

This product gave a positive signal in MCF7 fixed with 100% methanol (5 min).

Associated Products

Select an associated product type

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8 products for Alternative Version

Target data

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed:29499166, PubMed:30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

Alternative names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

Recommended products

Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to Alexa Fluor® 555. Suitable for mIHC, ICC and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1845Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.

Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Associated diseases and disorders

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Immunocytochemistry - Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883), expandable thumbnail

    Immunocytochemistry - Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883)

    Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling Sodium Potassium ATPase with ab274883.

    The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were tehn incubated with ab274883 at 1/100 dilution, overnight at +4°C (Green) and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal to alphaTubulin (Alexa Fluor® 647), at 1/250 dilution (Red). Nuclear DNA was labelled with DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883), expandable thumbnail

    Multiplex immunohistochemistry - Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883)

    Immunofluorescence analysis of human cervical squamous cell carcinoma labelled with ab274883. Image was acquired using Cell DIVE Multiplexed Imaging Solution (Leica Microsystems).

    Formalin fixed paraffin embedded (FFPE) sections were incubated with ab274883 at 1/100 dilution, for 1h at room temperature. Nuclear DNA was labelled with DAPI (Red). Deparaffinization, antigen retrieval, biomarker labeling and imaging was performed following Cell DIVE recommended standard protocol. Cell DIVE automatically processed the image to perform autofluorescence removal, distortion correction, blank glass subtraction, flat-field correction, and stitching.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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