Rabbit Monoclonal SOX10 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
ICC/IF | Flow Cyt (Intra) | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Tested |
Rat | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species Mouse | Dilution info 1/50 | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/500 | Notes - |
Species Mouse | Dilution info 1/50 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).
Transcription factor SOX-10, SOX10
Rabbit Monoclonal SOX10 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
SP267
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized B16F0 (mouse melanoma epithelial cell-like) cells labeling SOX10 with ab307763 at 1/50 dilution (1.0µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208569) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) (Left panel) / A375 (human malignant melanoma epithelial cell) (Right panel) cells labeling SOX10 with ab307763 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208569) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Negative control: HeLa.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling SOX10 with ab307763 at 1/50 dilution (10.0 µg/ml).
Confocal image showing nuclear staining in C6 cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized B16-F0 (mouse melanoma epithelial-like cell) cells labeling SOX10 with ab307763 at 1/50 dilution (10.0 µg/ml).
Confocal image showing nuclear staining in B16-F0 cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labeling SOX10 with ab307763 at 1/50 dilution (10.0 µg/ml).
Confocal image showing nuclear staining in A375 cells.
Negative control: HeLa (PMID:29315345).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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